Results 41 to 50 of about 3,785 (151)

DNA Library Prep for BGISEQ-500/MGISEQ-2000/DNBSEQ-G400/MGISEQ-200/DNBSEQ-G50 v1

, 2021
This DNA library prep protocol is specifically designed for WGS library construction for MGI highthroughput sequencing platforms. It's applicable for samples from all common animals, plants, fungi bacteria, etc., including human, mice, rice, Arabidopsis, yeast, E. coli, and Metagenomics samples.
openaire   +1 more source

In‐Tumor CRISPR‐Cas9 Knockout Screening and Novel Therapy Development for Malignant Transformation of Ovarian Teratoma

Cancer Science, EarlyView.
We conducted an in vivo genome‐wide CRISPR‐Cas9 knockout screen using MTMCT‐derived cells to identify genes essential for tumor growth. SOD1 emerged as a key therapeutic target, and its inhibition suppressed tumor proliferation by disrupting redox homeostasis, both in vitro and in PDX models. ABSTRACT Malignant transformation of mature cystic teratoma (
Satoshi Tamauchi, Kosuke Yoshida, Wang Xinyuan, Atsushi Nakagawa, Akira Yokoi, Nobuhisa Yoshikawa, Kaoru Niimi, Yusuke Yamamoto, Hiroaki Kajiyama   +8 more
wiley   +1 more source

Genome‐Wide Analysis Successfully Resolves Population Structure Shaped by Recent Divergence in the Endangered Bagrid Catfish Pseudobagrus ichikawai

Ecology and Evolution, Volume 16, Issue 4, April 2026.
Using genome‐wide SNP data, we revealed previously undetected population structure in the endangered bagrid catfish Pseudobagrus ichikawai. Our results suggest that past paleo‐river systems shaped the current genetic connectivity across the Ise Bay area, while low genetic diversity indicates historical bottlenecks and recent inbreeding.
Keisuke Onuki, Ryoichi Tabata, Tappei Mishina, Mutsumi Nishida, Katsutoshi Watanabe   +4 more
wiley   +1 more source

Balmer Lines as Diagnostics of Physical Conditions in AGN Broad Emission Line Regions

, 2003
Using a well known method for laboratory plasma diagnostic, the Boltzmann-plot, we discuss the physical properties in Broad Line Region (BLR) of Active Galactic Nuclei (AGN).
Popovic, L. C.
core   +2 more sources

Commitment to Myogenic Differentiation Significantly Aggravates the RNA Phenotype in Myotonic Dystrophy Type 1

Neuropathology and Applied Neurobiology, Volume 52, Issue 2, April 2026.
DM1 myoblasts show mild defects, but RNA toxicity intensifies upon differentiation, where broad gene‐expression changes and escalating MBNL1‐driven splicing defects disrupt muscle‐specific pathways, underscoring a key vulnerability at the transition from myogenic precursor cells to myofibres in patients. ABSTRACT Aims Myotonic dystrophy type 1 (DM1) is
Lise Ripken, Walther J. A. A. van den Broek, Remco T. P. van Cruchten, Jos G. A. Smits, Tabea V. Riepe, Peter A. C. 't Hoen, Derick G. Wansink   +6 more
wiley   +1 more source

Cas9‐Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A‐To‐G Base Editing in Rice

Plant Biotechnology Journal, Volume 24, Issue 4, Page 2576-2591, April 2026.
ABSTRACT Adenine base editors (ABEs) produce precise A‐to‐G conversion in the genomic target sites without causing double‐strand breaks. However, the hyperactive adenosine deaminase TadA8e raises safety concerns on genome‐wide off‐target edits. We engineered 11 chimeric proteins for ABEs (CP‐ABEs) by embedding hyperactive TadA8e within Cas9 nickase to ...
Jianjian Hu, Xue Li, Yuhong Gao, Yifan Guo, Yini Liu, Chen Wang, Gencheng Xu, Chaoyue Du, Shijia Liu, Zhigang Zhao, Yihua Wang, Yufeng Wu, Xiaoou Dong, Chao Li, Jianmin Wan   +14 more
wiley   +1 more source

Analysis of potential helicopter vibration reduction concepts [PDF]

, 1985
Results of analytical investigations to develop, understand, and evaluate potential helicopter vibration reduction concepts are presented in the following areas: identification of the fundamental sources of vibratory loads, blade design for low vibration,
Davis, M. W., Landgrebe, A. J.
core   +1 more source

Short-period X-ray oscillations in super-soft novae and persistent SSS [PDF]

, 2015
Transient short-period
Beardmore, A. P., Dobrotka, A., Drake, J. J., Henze, M., Hirsch, M., Kretschmar, P., Kuulkers, E., Ness, J. -U., Osborne, J. P., Piro, A. L., Schwarz, G., Starrfield, S., Wilms, J.   +12 more
core   +2 more sources

Behavioral Phenotype and Neuropsychological Profile of an Adult With Smith‐Magenis Syndrome due to a Previously Unreported RAI1 Mutation: A Case Report

Clinical Case Reports, Volume 14, Issue 3, March 2026.
ABSTRACT Smith‐Magenis Syndrome (SMS) is an uncommon genetic disorder caused by microdeletions of chromosome 17p11.2 including the RAI1 gene, or loss‐of‐function mutations that directly affect RAI1. Due to the involvement of RAI1 in neurodevelopment, SMS leads to typical pathologic features in the behavioral and physical phenotype that must be ...
Edgar Andrés Chavarría‐Martínez, Julen Arellano‐Sandúa, Ruth Arias‐Hidalgo, Ana Patiño‐García, Adrián Cano‐Prous   +4 more
wiley   +1 more source

Soil Microbial Functions Indicate Persistent Agricultural Legacies and Potential Alternative States Following Restoration Plantings

Ecology and Evolution, Volume 16, Issue 3, March 2026.
Soil microbial functions are key indicators of ecosystem recovery but can be obscured by taxonomic redundancy. Using shotgun metagenomics, we show that while overall functional diversity remains stable across land conditions, functional composition shifts persist in restored soils, particularly in relation to phosphorus metabolism.
Shawn D. Peddle, Christian Cando‐Dumancela, Sofie Costin, Tarryn Davies, Michael P. Doane, Robert A. Edwards, Riley J. Hodgson, Siegfried L. Krauss, Craig Liddicoat, Martin F. Breed   +9 more
wiley   +1 more source