Results 81 to 90 of about 3,049 (113)

Characterization of an activating factor required for hydrolysis of G_m2 ganglioside catalyzed by hexosaminidase A

Canadian Journal of Biochemistry, 1977
Separation of the hexosaminidase A (EC 3.2.1.52) and B isozymes of human liver by ion-exchange chromatography results in recovery of greater than 80% of the activity in crude extracts when synthetic substrates are used to monitor enzyme activity. Only 15% of hexosaminidase activity toward the N-acetylgalactosaminyl (N-acetylneuraminyl) galactosyl ...
Peter Hechtman
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Enzyme-linked Immunosorbent Assay for the Ganglioside G_M2-Activator Protein. Screening of Normal Human Tissues and Body Fluids, of Tissues of G_M2Gangliosidosis, and for Its Subcellular Localization

Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1984
An enzyme-linked immunosorbent assay was developed for the quantitation of the human activator protein for ganglioside GM2 degradation by beta-N-acetyl-D-hexosaminidase A. With this assay, various tissues and body fluids of normal subjects and of patients with variant forms of GM2 gangliosidosis were screened for their GM2-activator content.
A, Banerjee, J, Burg, E, Conzelmann, M, Carroll, K, Sandhoff   +4 more
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Biochemical Characterization of the Cys¹³⁸Arg Substitution Associated with the AB Variant Form of G_M2 Gangliosidosis:  Evidence That Cys¹³⁸ Is Required for the Recognition of the G_M2 Activator/G_M2 Ganglioside Complex by β-Hexosaminidase A

Biochemistry, 1998
Bei Xie, Brigitte Rigat, Natasha Smiljanic-Georgijev, Huinan Deng, Don Mahuran   +4 more
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Incorporation of Asialo G_M2 and Gangliosides in Cell Surface of Cultured Metastatic and Nonmetastatic Balb/3T3 Cell Lines: Altered Adhesion to Substrate In Vitro and Subcutaneous Tumor Cell Take<xref ref-type="fn" rid="fn2">2</xref><xref ref-type="fn" rid="fn3">3</xref>

JNCI: Journal of the National Cancer Institute, 1981
Ganesa Yogeeswaran
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GM2-Ganglioside Metabolism In Situ in Mucolipidosis IV Fibroblasts

Neurochemical Research, 1999
Mucolipidosis IV (ML IV) is an inherited lysosomal disorder for which the primary biochemical defect has not been identified. In order to detect any defect in glycosphingolipid metabolism, we have examined the metabolism of sphingosine-labeled (3H)G(M2) in situ in fibroblasts from patients diagnosed with ML IV.
S, Raghavan, E, Leshinsky, E H, Kolodny
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Recombinant Ganglioside GM2 Synthase—Expression in Insect Cells and Enzyme Assay

2003
Publisher Summary This chapter investigates the recombinant ganglioside GM2 synthase; and describes the expression of murine β-1,4-N-acetylgalactosaminyltransferase both as a complete membrane-bound enzyme and as a soluble form in the baculovirus insect -cell expression system.
Wendeler, M., Reiländer, H., Hoernschemeyer, J., Schwarzmann, G., Kolter, T., Sandhoff, K.   +5 more
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Development of a human monoclonal antibody to ganglioside G(M2) with potential for cancer treatment.

Cancer research, 1997
A human B-lymphoblastoid cell clone, L55-81, that produces human monoclonal antibody (MAb) to ganglioside G(M2) was established from peripheral blood B lymphocytes of a melanoma patient. L55-81 secretes IgMkappa light chain antibody in a serum-free medium.
Y, Nishinaka, M H, Ravindranath, R F, Irie   +2 more
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Vaccination with a bivalent G(M2) and G(D2) ganglioside conjugate vaccine: a trial comparing doses of G(D2)-keyhole limpet hemocyanin.

Clinical cancer research : an official journal of the American Association for Cancer Research, 2001
Immunization with GMK vaccine (G(M2) ganglioside conjugated to keyhole limpet hemocyanin mixed with QS-21 adjuvant) induces anti-G(M2) antibodies in close to 100% of patients. We found previously that anti-G(D2) antibodies could be induced in some patients using G(D2)-keyhole limpet hemocyanin + QS-21 (GDK).
P B, Chapman, D, Morrisey, K S, Panageas, L, Williams, J J, Lewis, R J, Israel, W B, Hamilton, P O, Livingston   +7 more
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Characterization of phospholipase D activation by G[M2] ganglioside activator in a cell-free system

, 1999
均 陣内
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Accumulation of G[M2] Ganglioside in Niemann-Pick Disease Type C Fibroblasts

, 1997
矢野珠巨
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