Results 121 to 130 of about 21,743,586 (235)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Berliner Corset-Fabrik W & G Neumann, 90 eigene Spezialgeschäfte

open access: yes
BERLINER CORSET-FABRIK W & G NEUMANN, 90 EIGENE SPEZIALGESCHÄFTE Berliner Corset-Fabrik W & G Neumann, 90 eigene Spezialgeschäfte ( -
MR, W & G Neumann
core   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

open access: yesFEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton   +6 more
wiley   +1 more source

Brief von L. W. G. Tutt an Josef Steindl

open access: yes
BRIEF VON L. W. G. TUTT AN JOSEF STEINDL Brief von L. W. G. Tutt an Josef Steindl ([1]
Tutt, L. W. G.
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Letter from Thos. J. Henley to G. W. Manypenny with a letter from G. W. Dickenson & nine others, 1855

open access: yes, 2008
Enclosed a copy of a letter from G. W.
Henley, Thomas J. (Thomas Jefferson), 1810-1865
core   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

open access: yesFEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

La condena de la venganza privada tras la justicia punitiva. Contraste y continuidad entre La Orestíada de Esquilo y el derecho hegeliano

open access: yesIdeas y Valores, 2016
Se abordan dos formas de justicia en principio antagónicas: la retributiva, propia del mundo de los héroes y de la venganza antigua, y la punitiva, de tradición tanto antigua como moderna, que consiste en la racionalización de la violencia mediante el ...
Víctor Ibarra B.
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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