Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Manual for starch gel electrophoresis: A method for the detection of genetic variation [PDF]
, 1987 The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts ...
Aebersold, Paul B. +4 more
core
Determination of association constants between 5 '-guanosine monophosphate gel and aromatic compounds by capillary electrophoresis [PDF]
, 2013 Hydro gel formed by 5'-guanosine monophosphate (GMP) in the presence of a potassium ion is expected to exhibit interesting selectivity in capillary electrophoretic separations.
Alberty +32 more
core +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
The synthesis and the catalytic (catalase and tyrosinase) activities of amino acid copper complexes covalently grafted onto silica gel [PDF]
, 2006 In this work the synthesis, structure and certain catalytic properties of amino acid copper complexes covalently grafted onto silica gel are described.
Hernádi, Klára +3 more
core
Fingering to fracturing transition in a transient gel
, 2013 Fracture processes are ubiquitous in soft materials, even in complex fluids, subjected to stresses. To investigate these processes in a simple geometry, we use a model self-assembled transient gel and study the instability patterns obtained in a radial ...
Foyart, Guillaume +3 more
core +3 more sources
Reconstructing 3D x-ray CT images of polymer gel dosimeters using the zero-scan method [PDF]
, 2013 In this study x-ray CT has been used to produce a 3D image of an irradiated PAGAT gel sample, with noise-reduction achieved using the ‘zero-scan’ method.
Baldock C +16 more
core +2 more sources
Molecular Oncology, EarlyView.Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice +16 more
wiley +1 more source