Results 151 to 160 of about 1,334,151 (300)

A 3D Printed Mini-Gel Electrophoresis System for Rapid and Inexpensive DNA Nanoswitch Biosensing. [PDF]

ACS Meas Sci Au
Morya V, Hayden A, Zhou L, Cole D, Halvorsen K.   +4 more
europepmc   +1 more source

Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone

Advanced Science, EarlyView.
A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad, Anastasiia Steksova, Yuyang Tang, Leyao Huang, Alireza Velayati, Shengwei Zhang, Aditi Dey Poonam, Sina Jamalzadegan, Matthew Breen, Hongjie Chen, Mahsa Bagi, Guochun Jiang, Qingshan Wei   +12 more
wiley   +1 more source

SEP Gel Electrophoresis Kit [PDF]

This SEP kit offers a series of activities for learning the principles of electrophoresis. After learning the basic technique, students progress to using agarose gel electrophoresis to separate mixtures of molecules.
Science Education Partnership, (SEP)
core  

An Adipocyte MKK3–p38β Axis Preserves Insulin Responsiveness in Mice and Is Reciprocally Altered in Human Obesity

Advanced Science, EarlyView.
The adipocyte MKK3–p38β axis emerges as an adaptive stress‐response pathway during obesity. Human visceral adipose tissue analyses, adipocyte‐specific mouse models, and primary adipocytes show that the MKK3–p38β axis supports insulin responsiveness by restraining p70S6K‐dependent inhibitory IRS1 feedback and preserving AKT–GLUT4 signaling. This pathway
Edgar Bernardo, Nuria Matesanz, Jorge‐Luis Torres, Alba C. Arcones, Juan Ignacio Jiménez‐Loygorri, Alfonso Mora, Leticia Herrera‐Melle, Luis Leiva‐Vega, Maria Elena Rodriguez, Lourdes Hernández‐Cosido, Marta Leon, Paula Yong González, Teresa Durán, Tamara Parracho, Francisca Mulero, Rubén Nogueiras, J. Simon C. Arthur, Angel R. Nebreda, Roger J. Davis, Miguel Marcos, Guadalupe Sabio   +20 more
wiley   +1 more source

Molecular profiling of the single-cell proteome via gel electrophoresis and 3D single-molecule imaging. [PDF]

Nat Commun
Kamarulzaman L, Kim S, Hidaka T, Tsuchida M, Taniguchi Y.   +4 more
europepmc   +1 more source

Biofilm‐Binding Phages Enhance Biofilm Eradication by Synergistic Photothermal and Photodynamic Therapy

Advanced Science, EarlyView.
Engineered phage‐based trifunctional nanofibers are developed for synergistic biofilm eradication. Biofilm‐binding phages, armed with AuNPs and TCPP, serve as both warheads and scaffolds, endowing the phototherapy agents with biofilm‐anchoring ability. Upon light irradiation, AuNPs induce photothermal therapy, while TCPP generates 1O2 for photodynamic ...
Ying Cao, Tao Yang, Rui Wang, Hui‐Da Li, Xiao‐Yu Zhang, Feng Cheng, Ying Liu, Jian‐Hua Wang, Ting Yang, Chuanbin Mao   +9 more
wiley   +1 more source

Borax-based gel electrophoresis: A novel approach for RNA integrity analysis. [PDF]

PLoS One
Albaser A.
europepmc   +1 more source

Selective miRNA quantitation with high-temperature thermal gel electrophoresis. [PDF]

Anal Chim Acta, 2023
Cornejo MA, Linz TH.
europepmc   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Gm26550 Modulates Learning and Memory by Increasing IGF1 mRNA Expression and Stability in Nrf2−/− Mice

Advanced Science, EarlyView.
The present study shows that Nrf2 directly binds to the Gm26550 promoter, thereby activating Gm26550 transcription and increasing its expression. Mechanistically, Gm26550 promotes IGF1 expression by functionally antagonizing miR‐26a‐5p‐mediated repression and sequestering the RBP KHSRP, thereby enhancing hippocampal neuronal synaptic plasticity and ...
Hongfang Wang, Ziyao Wang, Dongyue Zuo, Zhaowen Su, Bowen Song, Jiamin Gao, Yizhou Zhang, Ruiting Zhao, Linjie Sun, Mengdi Li, Yirui Fan, Dandan Geng, Lei Wang   +12 more
wiley   +1 more source