Results 241 to 250 of about 1,334,151 (300)
Some of the next articles are maybe not open access.
Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2020Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R, Green, Joseph, Sambrook
openaire +2 more sources
Carbohydrate Gel Electrophoresis
2010Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) relies on derivatization of reducing ends of sugars with a fluorophore, followed by electrophoresis under optimized conditions in polyacrylamide gels. PACE is a sensitive and simple tool for studying polysaccharide structure or quantity and also has applications in the investigation ...
Goubet, Florence +2 more
openaire +5 more sources
Difference gel electrophoresis
PROTEOMICS, 2008AbstractDIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical ...
John F, Timms, Rainer, Cramer
openaire +2 more sources
Cold Spring Harbor Protocols, 2019
This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
Michael R, Green, Joseph, Sambrook
openaire +2 more sources
This protocol describes steps for the preparation and running of agarose gels and for staining and visualization of DNA in gels using three dyes: ethidium bromide, SYBR Gold, and SYBR Green 1.
Michael R, Green, Joseph, Sambrook
openaire +2 more sources
Nanocellulose for gel electrophoresis
Journal of Colloid and Interface Science, 2019Cellulose nanofibres produced by TEMPO-mediated oxidation can form gels. This study presents a proof-of-concept for gel electrophoresis with nanocellulose (NC).TEMPO-oxidised cellulose nanofibre dispersion is chemically cross-linked by inducing amide linkages to produce gel slabs for electrophoretic separation.
Llyza, Mendoza +3 more
openaire +2 more sources
The gel electrophoresis of DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract 1. 1. We have compared the electrophoretic mobility of linear duplex DNAs (bacteriophages T4, lambda, T7 and Φ29) and of circular duplex DNAs (replicative form DNA of bacteriophage ΦX174, bacteriophage PM 2 DNA and rat mtDNA) in agarose gels.
C, Aaij, P, Borst
openaire +2 more sources
2003
After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
openaire +3 more sources
After digestion of DNA with a restriction enzyme (Chapter 50), it is usually necessary, for both preparative and analytical purposes, to separate and visualize the products. In most cases, where the products are between 200 and 20,000 bp long, this is achieved by agarose gel electrophoresis.
openaire +3 more sources
Difference gel electrophoresis DIGE
Drug Discovery Today: Technologies, 2006Proteomics offers powerful technologies to assist in the discovery of targets for novel therapeutic agents, by allowing the investigation of changes in protein state between control and diseased tissue and biofluids. Difference gel electrophoresis coupled with mass spectrometry (DIGE/MS) is a technology used within proteomics that has demonstrated ...
Kathryn S, Lilley, David B, Friedman
openaire +2 more sources
Current Protocols in Molecular Biology, 1992
AbstractAgarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5‐ to 25‐kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample ...
openaire +5 more sources
AbstractAgarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5‐ to 25‐kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample ...
openaire +5 more sources
Electrophoresis and Gel Strength
Nature, 1954A CURIOUS phenomenon has been noted in recent work on the electrophoresis of sodium carrageenate. This polysaccharide occurs in the red seaweed, Chondrus crispus, and is composed mainly of d-galactose sulphate units linked in the 1–3 positions. Cations associated with the sulphate can become ionized to make the molecule a polyelectrolyte.
openaire +2 more sources