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Two-dimensional gel electrophoresis of membrane proteins
Biochemistry, 1976A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G F, Ames, K, Nikaido
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Gel electrophoresis in studies of protein conformation and folding
Analytical Biochemistry, 1984Electrophoresis through polyacrylamide gels is a useful method for distinguishing conformational states of proteins and analyzing the thermodynamic and kinetic properties of transitions between conformations. Although the relationship between protein conformation and electrophoretic mobility is quite complex, relative mobilities provide qualitative ...
D P, Goldenberg, T E, Creighton
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Gel electrophoresis of proteins
1992Abstract At one time or another during the course of protein analysis or purification, researchers are likely to make use of gel electrophoresis. All laboratories working with proteins have some capability for carrying out gel electrophoresis and all researchers have at least rudimentary knowledge of the technique. Gel electrophoresis
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PREPARATIVE ELECTROPHORESIS OF PROTEINS IN ACRYLAMIDE GELS*
Annals of the New York Academy of Sciences, 1964SUMMARY(1) A simple apparatus is described for adaption of the “disc” electrophoresis techniques to a preparative procedure that permits analytic and radioactive monitoring of the effluent fractions automatically.(2) The efficiency of the procedure for separating a model system consisting of ribonuclease, trypsin, and chymotrypsin has been demonstrated.
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The separation of membrane proteins by polyacrylamide gel electrophoresis
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1971A modified Takayama (K. Takayama, D. H. Mac Lennanan, A. Tzagoloff and C. D. Stoner, Arch. Biochem. Biophys., 114 (1966) 223.) method for acrylamide gel electrophoresis of membrane proteins is described. The method uses acetic acid, urea, phenol and mercaptoethanol as the solvent for solubilizing the membranes.
T K, Ray, G V, Marinetti
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Gel Electrophoresis of Proteins
1998Abstract This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis ...
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Studies of DNA‐protein interactions by gel electrophoresis
ELECTROPHORESIS, 1989AbstractThe use of gel electrophoresis in studies of nucleic acid‐protein (especially DNA‐protein) interactions has yielded much qualitative and quantitative information about a variety of such systems. The reduction in mobility of complexes relative to free DNA allows isolation and characterization of the complexes as well as determination of ...
J A, Ceglarek, A, Revzin
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Gel Electrophoresis of Proteins and Enzymes
1976Proteins are defined as large molecular weight substances (104 – 105) composed of amino acids coupled by the α carboxyl group of one acid and the α amino group of another forming a polypeptide:
P. P. Feret, F. Bergmann
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Artifacts and Common Errors in Protein Gel Electrophoresis
2018In spite of taking precautions, some common mistakes creep into well-planned gel electrophoresis experiments. This occurs commonly in relation to calculating the cross-linking factor of a gel, polymerization temperature and time for a polyacrylamide gel, inducing aggregates in samples for electrophoresis, titrating the running buffer in electrophoresis,
Biji T, Kurien, R Hal, Scofield
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