Results 301 to 310 of about 864,514 (341)

Agarose native gel electrophoresis of proteins

International Journal of Biological Macromolecules, 2019
We have developed an agarose-based native gel electrophoresis system that works for both acidic and basic proteins using histidine-MES buffer. This electrophoresis can be done in a flat-bed mode or a vertical mode. While in the flat-bed mode both acidic and basic proteins can be simultaneously analyzed, the vertical gel can only be used for either ...
Cynthia, Li, Tsutomu, Arakawa
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SDS-Polyacrylamide Gel Electrophoresis of Proteins

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide.
Joseph, Sambrook, David W, Russell
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Supramolecular Gel Electrophoresis of Acidic Native Proteins

Analytical Chemistry, 2014
Amphiphilic tris-urea molecules self-assemble into a supramolecular hydrogel in tris(hydroxymethyl)aminomethane-glycine buffer. The supramolecular hydrogel is used as a matrix for the electrophoresis of acidic native proteins, in which proteins are separated based on their isoelectric points rather than their molecular weights.
Kanako, Munenobu, Takayuki, Hase, Takanori, Oyoshi, Masamichi, Yamanaka   +3 more
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Two-dimensional gel electrophoresis of membrane proteins

Biochemistry, 1976
A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G F, Ames, K, Nikaido
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Agarose-acrylamide gradient gel electrophoresis of proteins

Analytical Biochemistry, 1982
Abstract A new agarose-acrylamide gradient slab gel electrophoresis system is described. The preparation of this new gel has been facilitated by the use of agarose with a relatively low gelation temperature. Fractionation of marker proteins and crosslinked proteins from a subcellular cytoskeletal preparation on agarose-acrylamide gradient gels is ...
D F, Warren, M A, Naughton, L M, Fink
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Discontinuous gel electrophoresis of reduced membrane proteins

Biochemical and Biophysical Research Communications, 1974
Summary Fractionation of reduced membrane proteins in discontinuous sodium dodecyl sulfate gel electrophoresis is affected by reconstitution of disulfide bridges, by dissociation of dodecyl sulfate-protein complexes through sieving in the gel matrix, and by changes in mobility due to alterations in ionic environment of the sample.
C, Richter-Landsberg, R, Rüchel, T V, Waehneldt   +2 more
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Starch Gel Electrophoresis of Proteins

2003
Starch gel is one of a wide variety of supporting media that can be used for horizontal zone electrophoresis. Such gels are prepared by heating and cooling a quantity of partially hydrolyzed starch in an appropriate buffer solution. The choice of buffer is somewhat empirical and a wide variety of compositions have been used successfully.
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Two-dimensional gel electrophoresis of proteins

Journal of Chromatography B: Biomedical Sciences and Applications, 1987
The high-resolution capacity of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) makes it an excellent tool for the analysis and characterisation of complex protein mixtures. The evolution of two-dimensional electrophoresis is briefly described.
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SDS Polyacrylamide Gel Electrophoresis of Proteins

2003
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
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Gel electrophoresis of proteins

1992
Abstract At one time or another during the course of protein analysis or purification, researchers are likely to make use of gel electrophoresis. All laboratories working with proteins have some capability for carrying out gel electrophoresis and all researchers have at least rudimentary knowledge of the technique. Gel electrophoresis
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