Results 301 to 310 of about 856,230 (338)

Agarose native gel electrophoresis of proteins

International Journal of Biological Macromolecules, 2019
We have developed an agarose-based native gel electrophoresis system that works for both acidic and basic proteins using histidine-MES buffer. This electrophoresis can be done in a flat-bed mode or a vertical mode. While in the flat-bed mode both acidic and basic proteins can be simultaneously analyzed, the vertical gel can only be used for either ...
Cynthia, Li, Tsutomu, Arakawa
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Rapid capillary gel electrophoresis of proteins

Journal of Chromatography A, 1993
The rapid separation of sodium dodecyl sulphate-protein complexes according to their molecular masses (M(r)) by capillary gel electrophoresis is described. Using commercial equipment, standard proteins with M(r) in the range 29,000-97,400 were resolved to the baseline in less than 2 min by utilizing a separation distance of 7 cm.
R, Lausch, T, Scheper, O W, Reif, J, Schlösser, J, Fleischer, R, Freitag   +5 more
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Starch-Gel Electrophoresis of Wheat Proteins

Nature, 1960
IT has been claimed that starch-gel electrophoresis may have a higher resolving power than that of the Tiselius apparatus for serum proteins1. In spite of its potential value, there have been no reports in the literature of the application of this technique to the study of wheat proteins.
G A, ELTON, J A, EWART
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Starch Gel Electrophoresis of Proteins

2003
Starch gel is one of a wide variety of supporting media that can be used for horizontal zone electrophoresis. Such gels are prepared by heating and cooling a quantity of partially hydrolyzed starch in an appropriate buffer solution. The choice of buffer is somewhat empirical and a wide variety of compositions have been used successfully.
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SDS-Polyacrylamide Gel Electrophoresis of Proteins

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide.
Joseph, Sambrook, David W, Russell
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Two-dimensional gel electrophoresis of proteins

Journal of Chromatography B: Biomedical Sciences and Applications, 1987
The high-resolution capacity of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) makes it an excellent tool for the analysis and characterisation of complex protein mixtures. The evolution of two-dimensional electrophoresis is briefly described.
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Gel electrophoresis of proteins

1992
Abstract At one time or another during the course of protein analysis or purification, researchers are likely to make use of gel electrophoresis. All laboratories working with proteins have some capability for carrying out gel electrophoresis and all researchers have at least rudimentary knowledge of the technique.
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The interaction of proteins during gel electrophoresis

Journal of the Science of Food and Agriculture, 1966
AbstractReversible polymerisation and A + B ⇌ C type reactions among proteins during zone electrophoresis may give rise to tailing, extra bands and mobility changes. One or more of these signs are likely to appear with the milder type of interaction, i.e. equilibrium constant in the range 102–108, whether the time to equilibrium is measured in hours or
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A gel eluter for recovery of proteins separated by polyacrylamide gel electrophoresis

Analytical Biochemistry, 1982
Abstract An apparatus for the electrophoretic elution of proteins from polyacrylamide gel slices is described. The eluter is inexpensive, easy to build, and can be constructed to fit many available slab-gel apparatuses. A high yield of protein, concentrated in a small volume of solution, can be electrophoretically separated and recovered within a 24 ...
G L, Gerton, N J, Wardrip, J L, Hedrick
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Separation of olive proteins by capillary gel electrophoresis

Talanta, 2012
Olive proteins are not well known and there are still a lot of unknown information requiring further studies focused on the determination and characterization of these proteins. Despite the widely use of gel electrophoresis, this is the first time that capillary gel electrophoresis (CGE) is applied to separate proteins extracted from olive fruits ...
Cristina, Montealegre, Maria Concepción, García, Carmen, del Río, Maria Luisa, Marina, Carmen, García-Ruiz   +4 more
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