Cloning and characterisation of SSV genes from Saccharomyces Cerevisiae [PDF]
, 1995 In order to investigate the pleiotropic effects exhibited by mutations of S. cerevisiae defective in genes involved in vacuole biogenesis, a number of mutants were chosen for further study.
Hartley, Caroline Anne
core
Faster Bacterial Gene Cloning Using the Brick into the Gateway (BiG) Protocol.
Bio Protoc, 2022 Pierdoná FG +4 more
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Dead-End (dnd) Gene Cloning and Gonad-Specific Expression Pattern in Starry Flounder (Platichthys stellatus). [PDF]
Animals (Basel), 2021 Yoon JH +4 more
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Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
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NosZ gene cloning, reduction performance and structure of Pseudomonas citronellolis WXP-4 nitrous oxide reductase. [PDF]
RSC Adv, 2022 Hu L +7 more
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FEBS Open Bio, EarlyView.The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Genome-wide identification, gene cloning, subcellular location and expression analysis of SPL gene family in P. granatum L. [PDF]
BMC Plant Biol, 2021 Li B +6 more
europepmc +1 more source
From clones of cells to clones of genes.
Folia biologica, 1995 Three approaches to study the immune system are presented. First is the limiting dilution analysis, second is the analysis of the polypeptide patterns of lymphocytes by two-dimensional gel electrophoresis, and the third is the utilization of a partitioned cDNA library for establishing a gene catalog and proteinpaedia.
openaire +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source