Results 151 to 160 of about 13,121 (177)
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[MRSA--detection of mecA and its regulatory genes].
Rinsho byori. The Japanese journal of clinical pathology, 1994Methicillin resistance in S.aureus and S.epidermidis strains is primarily due to production of a new penicillin-binding protein PBP2' with extremely low binding affinity to most beta-lactam antibiotics. The structural gene for PBP2', mecA, is detectable in clinical specimens by using the polymerase chain reaction (PCR).
S, Kagawa, K, Yamashita, A, Matsuoka
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[Combination PCR of mecA, femA genes for detection of MRSA].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2004To make a comparison of PCR assay, Agar Dilution and Disk Diffusion as for detecting Staphylococcal mecA, femA genes.A total of 178 strains of Staphylococci were isolated from three large scale hospitals in Chengdu. Disk Diffusion and staphylococcal mecA, femA gene PCR assay for detecting MRSA were compared with Agar Dilution.The sensitivity and ...
Zhihong, Chen, Xinjian, Fan, Xiaoju, Lü
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Biosensors and Bioelectronics, 2018
Electrochemical DNA biosensor has unique advantages for on-site pathogenic microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) analysis remains challenge. In this work, we report a novel electrochemical biosensor for the ultrasensitive analysis of mecA DNA on methicillin-resistant Staphylococcus aureus (MRSA) genome, using ...
Li, Xu +8 more
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Electrochemical DNA biosensor has unique advantages for on-site pathogenic microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) analysis remains challenge. In this work, we report a novel electrochemical biosensor for the ultrasensitive analysis of mecA DNA on methicillin-resistant Staphylococcus aureus (MRSA) genome, using ...
Li, Xu +8 more
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Rapid Detection of the Methicillin-resistance Gene,mecA, in Coagulase-negative Staphylococci
Scandinavian Journal of Infectious Diseases, 2001Phenotypical methods are routinely used to detect methicillin resistance in Staphylococci. These methods are time-consuming and there are difficulties in detecting all resistant strains carrying the mecA gene. We detected methicillin-resistant Staphylococci in biological samples by PCR amplification of mecA, without the time-consuming step of ...
T, Glad +4 more
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Staphylococcus pseudintermedius heterogeneously expresses the mecA gene
Veterinary Microbiology, 2013Vincenzo, Savini +5 more
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[The structure and function of the mecA gene in methicillin-resistant Staphylococcus aureus].
Nihon rinsho. Japanese journal of clinical medicine, 1992Penicillin binding protein (PBP) 2' is the most important mechanism of the resistance to beta-lactams in methicillin-resistant Staphylococcus aureus (MRSA). And the mecA gene is the coding gene of PBP2', and located in the SmaI fragment G of the chromosome map by Pattle P.A.,. A part of the structure of mecA is similar to that of the penicillinase gene.
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[Analysis of the relationship between the MecA gene and resistance of β-lactam antibiotics].
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 2014To investigate the mechanisms by which MecA gene expression leads to β-lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA), and to study the resistance mechanism of MRSA at the molecular level.A variety of molecular biological techniques were employed, including screening MRSA using cefoxitin paper disk method, extraction of MRSA ...
Hui, Huang +3 more
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