Results 191 to 200 of about 47,017 (222)
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Rapid identification of bacteria, mecA and van genes from blood cultures
Pathologie Biologie, 2007The Genotype technology, a quick molecular genetic assay based on DNA multiplex amplification with biotinylated primers followed by hybridization to membrane bound probes, complies with the requirements for a fast diagnosis of sepsis. We evaluated the new Genotype BC Gram-negative and Gram-positive test kits (Hain Life Science, Germany) which ...
M-F, Prère, O, Baron, O, Fayet
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Biosensors and Bioelectronics, 2022
An outstanding dual-labeling ratiometric electrochemical biosensor based on isothermal strand displacement polymerization reaction (ISDPR) for highly sensitive and selective detection of mecA gene has been proposed. Concretely, in the presence of mecA gene, the addition of methylene blue (MB)-labeled primer and polymerase induced recycling ...
Yongxin Zhai +3 more
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An outstanding dual-labeling ratiometric electrochemical biosensor based on isothermal strand displacement polymerization reaction (ISDPR) for highly sensitive and selective detection of mecA gene has been proposed. Concretely, in the presence of mecA gene, the addition of methylene blue (MB)-labeled primer and polymerase induced recycling ...
Yongxin Zhai +3 more
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The Molecular Detection of mecA Genes of Staphylococcus Aureus
Annals of Advanced Biomedical Sciences, 2019Antibiotic resistance is common among pathogenic bacteria associated with community acquired and nosocomial infections. Methicillin-resistant Staphylococcus aureus (MRSA) infections have become a global health problem particularly in hospital setup causing simple skin infections to life threatening infections. The present study aimed to investigate the
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[MRSA--detection of mecA and its regulatory genes].
Rinsho byori. The Japanese journal of clinical pathology, 1994Methicillin resistance in S.aureus and S.epidermidis strains is primarily due to production of a new penicillin-binding protein PBP2' with extremely low binding affinity to most beta-lactam antibiotics. The structural gene for PBP2', mecA, is detectable in clinical specimens by using the polymerase chain reaction (PCR).
S, Kagawa, K, Yamashita, A, Matsuoka
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Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections.
Pakistan journal of pharmaceutical sciences, 2019It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin
Karthikeyan, Vijayan +3 more
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Rapid Detection of the Methicillin-resistance Gene,mecA, in Coagulase-negative Staphylococci
Scandinavian Journal of Infectious Diseases, 2001Phenotypical methods are routinely used to detect methicillin resistance in Staphylococci. These methods are time-consuming and there are difficulties in detecting all resistant strains carrying the mecA gene. We detected methicillin-resistant Staphylococci in biological samples by PCR amplification of mecA, without the time-consuming step of ...
T, Glad +4 more
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[Combination PCR of mecA, femA genes for detection of MRSA].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2004To make a comparison of PCR assay, Agar Dilution and Disk Diffusion as for detecting Staphylococcal mecA, femA genes.A total of 178 strains of Staphylococci were isolated from three large scale hospitals in Chengdu. Disk Diffusion and staphylococcal mecA, femA gene PCR assay for detecting MRSA were compared with Agar Dilution.The sensitivity and ...
Zhihong, Chen, Xinjian, Fan, Xiaoju, Lü
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Staphylococcus pseudintermedius heterogeneously expresses the mecA gene
Veterinary Microbiology, 2013Vincenzo, Savini +5 more
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