RNA‐Seq for Bacterial Gene Expression [PDF]
Current Protocols in Nucleic Acid Chemistry, 2018 AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
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VBCG: 20 validated bacterial core genes for phylogenomic analysis with high fidelity and resolution
Microbiome, 2023 Background Phylogenomic analysis has become an inseparable part of studies of bacterial diversity and evolution, and many different bacterial core genes have been collated and used for phylogenomic tree reconstruction.
Renmao Tian, Behzad Imanian
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Gene Location and Bacterial Sequence Divergence [PDF]
Molecular Biology and Evolution, 2002 Previous comparison of a relatively small set of homologous genes from Escherichia coli and Salmonella typhimurium revealed that genes nearer to the origin of replication had substitution rates lower than genes closer to the replication terminus. The recently completed sequences of numerous bacterial genomes have allowed us to test whether this effect ...
Mira A., Ochman H.
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Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia [PDF]
, 2010 In this study, the combination of culture enrichments and molecular tools was used to identify bacterial guilds, plasmids and functional genes potentially important in the process of petroleum hydrocarbon (PH) decontamination in mangrove microniches ...
Baek +81 more
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Sugar Lego: gene composition of bacterial carbohydrate metabolism genomic loci
Biology Direct, 2017 Background Bacterial carbohydrate metabolism is extremely diverse, since carbohydrates serve as a major energy source and are involved in a variety of cellular processes.
Anna Kaznadzey +2 more
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The coevolution of toxin and antitoxin genes drives the dynamics of bacterial addiction complexes and intragenomic conflict [PDF]
, 2012 Bacterial genomes commonly contain ‘addiction’ gene complexes that code for both a toxin and a corresponding antitoxin. As long as both genes are expressed, cells carrying the complex can remain healthy.
Brown, Sam P. +3 more
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Gene therapy using bacterial vectors
Frontiers in Bioscience, 2017 Bacteria can be used for gene therapy via two strategies - either by transfection of eukaryotic host cells using bacteria (bactofection) or by alternative gene therapy that does not alter the host genome, but uses the prokaryotic expression system, which can be controlled or stopped from outside.
Peter, Celec, Roman, Gardlik
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Genome wide expression profiling reveals suppression of host defence responses during colonisation by Neisseria meningitides but not N. lactamica. [PDF]
, 2011 Both Neisseria meningitidis and the closely related bacterium Neisseria lactamica colonise human nasopharyngeal mucosal surface, but only N. meningitidis invades the bloodstream to cause potentially life-threatening meningitis and septicaemia.
Hibberd, Martin L +4 more
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Enzymes are enriched in bacterial essential genes.
PLoS ONE, 2011 Essential genes, those indispensable for the survival of an organism, play a key role in the emerging field, synthetic biology. Characterization of functions encoded by essential genes not only has important practical implications, such as in identifying
Feng Gao, Randy Ren Zhang
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Increased variability of microbial communities in restored salt marshes nearly 30 years after tidal flow restoration [PDF]
, 2012 We analyzed microbial diversity and community composition from four salt marsh sites that were impounded for 40–50 years and subsequently restored and four unimpounded sites in southeastern Connecticut over one growing season.
Bernhard, Anne E +2 more
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