Results 191 to 200 of about 469,914 (305)

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Genetic engineering in potato. [PDF]

, 1988
Ooms, G.
core  

A modular protein-nucleic acid nanodevice for non-genetic engineering of mesenchymal stem cells to target pathological collagen in liver fibrosis. [PDF]

J Nanobiotechnology
Tian F, Wang K, Zheng C, Liu X, Shi P, Wang Z, Zhang T, Chen B, Wang F, Wang H, Nie Z, Huang Y.   +11 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Establishment of a HicA toxin-based counterselection system for markerless genetic engineering in Veillonella atypica OK5. [PDF]

BMC Microbiol
Zhou P.
europepmc   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Genomics, genetic engineering and artificial cells

Annals of Indian Academy of Neurology, 2010
Thomas Sanjeev
doaj  

From Genetic Engineering to Preclinical Safety: A Study on Recombinant Human Interferons. [PDF]

Int J Mol Sci
Ramos TI, Villacis-Aguirre CA, Lamazares E, Manrique-Suárez V, Sandoval F, Culqui-Tapia CN, Martin-Solano S, Mansilla R, Cabezas I, Sánchez O, Donoso-Erch S, Parra NC, Contreras MA, Santiago-Vispo N.   +13 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

GENETIC ENGINEERING: PRINCIPLES AND METHODS, VOLUME 3 [PDF]

, 1981

core