Results 321 to 330 of about 5,464,915 (400)
Intein‐based modular chimeric antigen receptor platform for specific CD19/CD20 co‐targeting
Molecular Oncology, EarlyView.CARtein is a modular CAR platform that uses split inteins to splice antigen‐recognition modules onto a universal signaling backbone, enabling precise, scarless assembly without re‐engineering signaling domains. Deployed here against CD19 and CD20 in B‐cell malignancies, the design supports flexible multi‐antigen targeting to boost T‐cell activation and
Pablo Gonzalez‐Garcia +9 more
wiley +1 more source
Current trends in single‐cell RNA sequencing applications in diabetes mellitus
FEBS Open Bio, EarlyView.Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Two‐way inhibition of PAX5 transcriptional activity by PAX5::CBFA2T3
FEBS Open Bio, EarlyView.PAX5::CBFA2T3 (PAX5‐C) is a fusion protein of the B‐cell transcription factor, PAX5, and is found in B‐cell ALL. We propose a putative model of two‐way inhibition of PAX5 transcriptional activity by PAX5‐C. There are two ways of repression by PAX5‐C: DNA‐binding‐dependent way and HDAC‐dependent way, with either being sufficient for the repression. HDAC
Reina Ueno +12 more
wiley +1 more source
Ro 31‐8220 suppresses bladder cancer progression via enhancing autophagy in vitro and in vivo
FEBS Open Bio, EarlyView.The pan‐protein kinase C inhibitor Ro‐31‐8220 demonstrates potent anti‐bladder cancer effects both in vitro and in vivo by suppressing migration/invasion, inducing apoptosis and crucially activating autophagy, where blocking autophagy with chloroquine reduces its cell‐killing efficacy, suggesting its promise as a novel therapeutic candidate requiring ...
Shengjun Fu +12 more
wiley +1 more source
FEBS Open Bio, EarlyView.In this research, a rat model of asthma was created using OVA, and polydatin served as an intervention. By inhibiting ferroautophagy mediated by NCOA4 and averting ferroptosis, polydatin has been demonstrated to reduce asthma. This work presents new ideas for investigating the mechanism of polydatin's ability to alleviate asthma, in addition to ...
Wei Li +5 more
wiley +1 more source
High LRIG1 expression predicts lymph node metastasis in patients with uterine cervical cancer
FEBS Open Bio, EarlyView.Lymph node status is crucial in determining treatment for women with early‐stage cervical cancer. We demonstrate that high LRIG1 protein expression in primary tumors can predict lymph node metastases. Our findings support further investigation of LRIG1 as a biomarker to improve staging accuracy and guide treatment decisions in cervical cancer patients.
Pernilla Israelsson +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study presents a novel approach to teaching Python and bioinformatics using team‐based learning and cloud‐hosted notebooks. By integrating interactive coding into biomedical education, the method improves accessibility, student engagement, and confidence—especially for those without a computing background.
Nuno S. Osório, Leonardo D. Garma
wiley +1 more source