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Conservation Genetics: Techniques and Fundamentals
Ecological Applications, 1992Conservation genetics utilizes the tools and concepts of genetics and applies them to problems in conservation biology. For example, molecular genetic techniques, such as protein electrophoresis, and analysis of mitochondrial DNA and highly variable nuclear genes (including DNA fingerprinting), have been important in documenting the extent and pattern ...
Philip W, Hedrick, Philip S, Miller
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Genetic engineering Techniques
1997This chapter gives a brief introduction to the biotechnological techniques which have been used for transforming genomes of plants and plastids. The aim of the engineering methods is to transfer a piece of a species-alien DNA molecule into the genome of a selected species providing the species with a new function or an altered function.
Simonsen, V., Jørgensen, R. B.
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Genetic Techniques in Trypanosoma cruzi
2011It is almost 20 years since genetic manipulation of Trypanosoma cruzi was first reported. In this time, there have been steady improvements in the available vector systems, and the applications of the technology have been extended into new areas.
Martin C, Taylor +2 more
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Techniques for Genetic Manipulation
In the last few decades, molecular techniques and genetic modification have been used in genotype and phenotype studies of S. suis. Genomic modification of S. suis requires DNA acquisition and its stable insertion into the chromosome by allelic exchange. In this chapter, we described two techniques for the preparation of genomic constructs (cloning andCarla, García López +6 more
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Genetic Manipulation Techniques
2014In the past two decades we have seen a very important advance in the research of the biology of Entamoeba histolytica. This dramatic progress has been mostly the result of (1) the introduction of a transfection system to express exogenous genes and to up- and downregulate the expression of selected genes, (2) the completion of the parasite’s genome ...
David Mirelman, Rivka Bracha
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2020
A modern genomic laboratory will offer a range of diagnostic techniques. A biochemical genetic laboratory may also be based in selected centres. In this chapter, a brief account of diagnostic techniques that are useful for inherited cardiac diseases is provided. It covers variant classification and techniques that can detect DNA sequence variants, next
Perry Elliott +2 more
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A modern genomic laboratory will offer a range of diagnostic techniques. A biochemical genetic laboratory may also be based in selected centres. In this chapter, a brief account of diagnostic techniques that are useful for inherited cardiac diseases is provided. It covers variant classification and techniques that can detect DNA sequence variants, next
Perry Elliott +2 more
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An Overview of Molecular Genetic Diagnosis Techniques
Current Protocols in Human Genetics, 2020AbstractOur understanding of genetic disease(s) has increased exponentially since the completion of human genome sequencing and the development of numerous techniques to detect genetic variants. These techniques have not only allowed us to diagnose genetic disease, but in so doing, also provide increased understanding of the pathogenesis of these ...
Rashmi S, Goswami, Shuko, Harada
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Genetic ethics and mtDNA replacement techniques
The New Bioethics, 2021The United Kingdom parliament made history in 2015 when they voted to allow the use of mitochondrial (mtDNA) replacement techniques. Mitochondrial diseases are genetically inherited and currently, the only known cure is replacement therapies. However, most other countries have been slow to adopt policy changes that allow for mtDNA replacement due to ...
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Molecular Genetic Techniques and Applications in Ophthalmology
Seminars in Ophthalmology, 1995Genetic Disorders that affect ocular tissues are responsible for malformations of the eye, blindness, and pain. Fifty percent of all childhood blindness is attributable to genetic disorders.1 Improvements in our ability to locate and analyze genes have led to a revolutionary increase in our knowledge of genetic disorders.
L M, Mullen, K W, Small
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Genetic principles and techniques
2003Abstract Considerable progress has been made in defining the underlying genetic basis of many Mendelian and mitochondrial diseases which can cause stroke and other cerebrovascular diseases.
Andrew J Catto, Angela M Carter
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