Results 21 to 30 of about 1,035,101 (349)

Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method

open access: yesPlant Methods, 2023
Background Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem in modern biology. It is assumed
Yufei Xia   +7 more
doaj   +1 more source

Genetic Transformation in Yeasts [PDF]

open access: yesJournal of General Microbiology, 1974
SUMMARY: The transformability of 25 strains of yeasts belonging to 23 species in three genera - Saccharomyces, Hansenula and Candida - was investigated with respect to ten characters. Eighteen hour cultures at about the end of the log phase of growth, addition of 10 μg DNA/ml, and an incubation time of 30 min at 30 to 37 °C usually yielded best results.
N C, Khan, S P, Sen
openaire   +2 more sources

From “the dialectics of nature” to the inorganic gene [PDF]

open access: yes, 1999
The concept of projection from one space to another, with a consequent loss of information, can be seen in the relationships of gene to protein and language description to real situation.
Mackay, Alan L.
core   +1 more source

Marker-free transgenic plants through genetically programmed auto-excision [PDF]

open access: yes, 2007
We present here a vector system to obtain homozygous marker-free transgenic plants without the need of extra handling and within the same time frame as compared to transformation methods in which the marker is not removed.
Verweire, Dimitri   +4 more
core   +2 more sources

Genetic approaches in improving biotechnological production of taxanes: An update

open access: yesFrontiers in Plant Science, 2023
Paclitaxel (PTX) and its derivatives are diterpene alkaloids widely used as chemotherapeutic agents in the treatment of various types of cancer. Due to the scarcity of PTX in nature, its production in cell cultures and plant organs is a major challenge ...
Edgar Perez-Matas   +6 more
doaj   +1 more source

Genetic Transformation in Rhizobium [PDF]

open access: yesJournal of General Microbiology, 1969
SUMMARY: Mutants of ground nut (Rhizobium cowpea group) strain gr3 were isolated using ultraviolet radiation and N-methyl-N'-nitrosoguanidine (NTG) as mutagenic agents. An adenine-requiring mutant was used as the recipient and its wild type as the donor in transformation studies. Initially minimal medium supplemented with 2 μg. adenine/ml. was used for
J L, Raina, V V, Modi
openaire   +2 more sources

Functional Characterization of Two Cell Wall Integrity Pathway Components of the MAPK Cascade in Phomopsis longicolla

open access: yesMolecular Plant-Microbe Interactions, 2023
The pathogenic fungus Phomopsis longicolla causes numerous plant diseases, such as Phomopsis seed decay, pod and stem blight, and stem canker, which seriously affect the yield and quality of soybean production worldwide.
Chen Zhang   +4 more
doaj   +1 more source

Continuous dynamic problem generators for evolutionary algorithms [PDF]

open access: yes, 2007
This article is posted here with permission from IEEE - Copyright @ 2007 IEEEAddressing dynamic optimization problems has attracted a growing interest from the evolutionary algorithm community in recent years due to its importance in the applications of ...
Tinos, R, Yang, S
core   +2 more sources

Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]

open access: yes, 2020
The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes   +5 more
core   +1 more source

Transcriptomic analysis of Eruca vesicaria subs. sativa lines with contrasting tolerance to polyethylene glycol-simulated drought stress

open access: yesBMC Plant Biology, 2019
Background Eruca vesicaria subsp. sativa is one of the Cruciferae species most tolerant to drought stress. In our previous study some extremely drought-tolerant/sensitive Eruca lines were obtained.
Bang-Lian Huang   +7 more
doaj   +1 more source

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