Results 181 to 190 of about 522,034 (344)

Liquid-phase imaging of bone development and calcification by atmospheric scanning electron microscopy (ASEM): Application to immuno-labeling and rapid tissue observation of genetically modified mouse [PDF]

, 2021
Eiko Sakai, Mari Sato, Nassirhadjy Memtily, Takayuki Tsukuba, Chikara Sato   +4 more
openalex   +1 more source

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

Advanced Functional Materials, EarlyView.
CRISPR machinery in diverse molecular formats (DNA, RNA, and ribonucleic protein) is complexed into nanoparticles with the cell‐friendly arginine‐alanine‐leucine‐alanine (RALA) cell‐penetrating peptide. Nanoparticles are delivered to primary mesenchymal stem cells ex vivo or locally in vivo to facilitate multimodal CRISPR gene editing. This RALA‐CRISPR
Joshua P. Graham, Jose Gabriel Castro, Lisette C. Werba, Joseph Amitrano, Luke Fardone, Kevin P. Francis, Anand Ramamurthi, Michael J. Layden, Helen O. McCarthy, Tomas Gonzalez‐Fernandez   +9 more
wiley   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Endocytic Programming via Porous Silicon Nanoparticles Enhances TLR4 Nanoagonist Potency for Macrophage‐Mediated Immunotherapy

Advanced Functional Materials, EarlyView.
Porous silicon nanoparticles (PSiNPs) reprogram macrophage endocytosis of manganese@albumin‐based TLR4 nanoagonists, driving TRIF‐biased TLR4 signaling, eliciting robust proinflammatory responses, and potentiating macrophage‐mediated immunotherapeutic effects against NSCLC.
Xiaomei Zhang, Huiying Li, Shuodan Huang, Linxuan Zhang, Yan Gao, Ruoran Wang, Xiaoyu Wang, Hélder A. Santos, Zhenyu Yin, Bing Xia   +9 more
wiley   +1 more source

The diversity of sex steroid action: novel functions of hydroxysteroid (17β) dehydrogenases as revealed by genetically modified mouse models.

Journal of Endocrinology, 2012
T. Saloniemi, Heli Jokela, L. Strauss, P. Pakarinen, M. Poutanen   +4 more
semanticscholar   +1 more source

Tunable Synthetic Hydrogel Modulates Hepatic Lineage Specification of Human Liver Organoid

Advanced Functional Materials, EarlyView.
In this study, a synthetic hydrogel is reported that supports the formation of hiPSC‐derived human liver organoids (HLOs). Hepatic lineage specification can be modulated via conjugation of RGD peptide to hydrogel: RGD‐conjugated hydrogels promote cholangiocyte differentiation, whereas RGD‐free hydrogels favor hepatocyte commitment of HLO cells.
Lei Wang, Lasse Riediger, Qi Rao, Xun Xu, Yan Nie, Yiduo Zhou, Junyao Zhang, Peng Tang, Weiwei Wang, Frank Tacke, Adrien Guillot, Ludovic Vallier, Irene Talon, Wenzhong Li, Yi‐An Yang, Rainer Haag, Nan Ma   +16 more
wiley   +1 more source

Altered balance of excitatory and inhibitory learning in a genetically modified mouse model of glutamatergic dysfunction relevant to schizophrenia. [PDF]

Sci Rep, 2017
Sanderson DJ, Lee A, Sprengel R, Seeburg PH, Harrison PJ, Bannerman DM.   +5 more
europepmc   +1 more source

3D Multicellular Scaffold Based Model for Advancing Bone Disorder Research

Advanced Functional Materials, EarlyView.
A scalable 3D multicellular in vitro bone model engineered by integrating osteoblasts, osteoclasts, and endothelial cells on biodegradable scaffolds. The system recapitulates key features of human bone remodeling and disease pathology. As a proof of concept, the model mimics osteogenesis imperfecta, demonstrating its potential as a physiologically ...
Gali Guterman‐Ram, Majd Machour, Janette Zavin, Roy Meretzki, Reut Lev‐Ari, Shulamit Levenberg   +5 more
wiley   +1 more source

Genetically Modified Mouse Models for Sarcoma Research: A Comprehensive Review. [PDF]

Curr Oncol Rep
Remiszewski P, Siedlecki E, Wełniak-Kamińska M, Mikula M, Czarnecka AM.   +4 more
europepmc   +1 more source

Altered expression of neuronal tryptophan hydroxylase-2 mRNA in the dorsal and median raphe nuclei of three genetically modified mouse models relevant to depression and anxiety

, 2011
Ali Jahanshahi, Erwan Le Maître, Yasin Temel, Laurence Lanfumey, M. Hamon, Klaus‐Peter Lesch, R.M. Tordera, Joaquı́n Del Rı́o, Ester Aso, Rafaël Maldonado, Tomas Hökfelt, Harry W.M. Steinbusch   +11 more
openalex   +2 more sources