Results 201 to 210 of about 247,762 (263)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Ethyl methanesulfonate mutagenesis in fungi: genetic mechanisms, applications, and implications for agricultural biotechnology. [PDF]

Arch Microbiol
Holman DE, Abrahams AI, Hattingh K, Saulse C, Hess M, Stegmann D, Roomanay M, Basson G, Klein A, Keyster M.   +9 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

A Comprehensive Analysis of the Agreement and Performance of Variant Annotation Programs in Equine Genomes. [PDF]

Genes (Basel)
Marlowe JL, Hughes L, Barrey E, Leeb T, Bellone R, McCue ME, Durward-Akhurst S.   +6 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Multi-ancestry polygenic risk scores for the prediction of type 2 diabetes and complications in diverse ancestries. [PDF]

Lancet Diabetes Endocrinol
Huerta-Chagoya A, Kim J, Mandla R, Lu Y, Suzuki K, Petty LE, Ng HK, Choi J, Lee S, Rout M, Lin K, Taylor K, ENSA Genomics Consortium, Genes & Health Research Team, VA Million Veteran Program, Aguilar-Salinas CA, García-García L, González-Villalpando C, Haiman CA, Kim YJ, Kwak SH, Leong A, Loos RJF, Moreno-Estrada A, Morris AP, Orozco L, Rotter JI, Sanghera D, Tusie-Luna T, Voight BF, Vujkovic M, Walters RG, Ge T, Manning AK, Loh M, Below JE, Sim X, Mercader JM, Ng MCY, D-PRISM Consortium.   +39 more
europepmc   +1 more source

Compressing the collective knowledge of ESM into a single protein language model. [PDF]

Nat Methods
Dinh T, Jang SK, Zaitlen N, Ntranos V.
europepmc   +1 more source

An increased number of heterozygous calls in the AxiomTM Equine Genotyping Array. [PDF]

G3 (Bethesda)
Gmel AI, Pirani A, McInnis L, Neuditschko M.   +3 more
europepmc   +1 more source

Polygenic Risk Scores for Breast Cancer Among African American Women With High Risk.

JAMA Netw Open
Sun Y, Simmons T, Li JL, Jamal A, Manirakiza AVC, Pruss D, Ratzel S, Olopade OI, Gutin A, Hughes E, Huo D.   +10 more
europepmc   +1 more source

A de novo start-loss variant in the NIPBL gene causing mild type 1 Cornelia de Lange syndrome in an Iranian family: A Case Report. [PDF]

J Med Case Rep
Mir A, Abbasi Z, Song Y, Lee H, Khajeh A, Jahantigh M, Hajigholami S, Khedri S, Tabatabaiefar MA.   +8 more
europepmc   +1 more source