Results 121 to 130 of about 4,164,752 (382)

Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM

Molecular Oncology, EarlyView.
CircRNAs are differentially expressed in glioblastoma primary tumors and might serve as therapeutic targets and diagnostic markers. The investigation of circRNA and RNA‐binding proteins (RBPs) interactions shows that distinct RBPs play a role in circRNA biogenesis and function.
Julia Latowska‐Łysiak, Żaneta Zarębska, Marcin P. Sajek, Adriana Grabowska, Alessia Buratin, Paweł Głodowicz, Julia O. Misiorek, Konrad Kuczyński, Stefania Bortoluzzi, Marek Żywicki, Jan G. Kosiński, Agnieszka Rybak‐Wolf, Rafał Piestrzeniewicz, Anna M. Barciszewska, Katarzyna Rolle   +14 more
wiley   +1 more source

Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. [PDF]

, 2015
Mouse embryonic stem cells (ESCs) are genetically more stable than somatic cells, thereby preventing the passage of genomic abnormalities to their derivatives including germ cells. The underlying mechanisms, however, remain largely unclear. In this paper,
Briggs, Steven P, Kim, Jinchul, Lee, Pei-Jen, Shen, Zhouxin, Su, Ning-Yuan, Todorova, Dilyana, Xiong, Jianhua, Xu, Yang   +7 more
core  

RNA polymerase V-dependent small RNAs in Arabidopsis originate from small, intergenic loci including most SINE repeats. [PDF]

, 2012
In plants, heterochromatin is maintained by a small RNA-based gene silencing mechanism known as RNA-directed DNA methylation (RdDM). RdDM requires the non-redundant functions of two plant-specific DNA-dependent RNA polymerases (RNAP), RNAP IV and RNAP V.
Chen, Xuemei, Gurazada, Sai Guna Ranjan, Lee, Tzuu-fen, Li, Shengben, Matzke, Marjori A, Meyers, Blake C, Simon, Stacey A, Zhai, Jixian   +7 more
core   +2 more sources

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A., Bahkali, A.H., Moslem, M.A., Wit, P.J.G.M., de   +3 more
core   +2 more sources

Elucidating prognostic significance of purine metabolism in colorectal cancer through integrating data from transcriptomic, immunohistochemical, and single‐cell RNA sequencing analysis

Molecular Oncology, EarlyView.
Low expression of five purine metabolism‐related genes (ADSL, APRT, ADCY3, NME3, NME6) was correlated with poor survival in colorectal cancer. Immunohistochemistry analysis showed that low NME3 (early stage) and low ADSL/NME6 (late stage) levels were associated with high risk.
Sungyeon Kim, Myunghee Kang, Soyeon Jeong, Jisup Kim, Kyoung Oh Kim, Won‐Suk Lee, Jeong‐Heum Baek, Jung Ho Kim, Seungyoon Nam   +8 more
wiley   +1 more source

Retrotransposon silencing by DNA methylation can drive mammalian genomic imprinting [PDF]

, 2015
Among mammals, only eutherians and marsupials are viviparous and have genomic imprinting that leads to parent-of-origin-specific differential gene expression. We used comparative analysis to investigate the origin of genomic imprinting in mammals. PEG10 (
Alsop, Amber, Graves, Jennifer, Ishino, Fumitoshi, Kaneko-Ishino, Tomoko, Kohara, Yuji, Kohda, Takashi, Narita, Takanori, Ono, Ryuichi, Pask, Andrew J, Renfree, Marilyn B, Shaw, Geoffrey, Suzuki, Shunsuke, Wang, Changshan   +12 more
core   +1 more source

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

DNA methylation and chromatin structure: The puzzling CpG islands [PDF]

, 2005
DNA methylation is the epigenetic modification,which introduces 5mC as fifth base onto DNA. As for the distribution of 5rnCs, it is well known that they distribute themselves in a non-random fashion in genomic DNA so that methylation pattern is ...
CAIAFA, Paola, ZAMPIERI, Michele
core   +1 more source

A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis.

Journal of Biomolecular Techniques, 2013
Isolation of DNA from blood and buccal swabs in adequate quantities is an integral part of forensic research and analysis. The present study was performed to determine the quality and the quantity of DNA extracted from four commonly available samples and
S. Ghatak, R. Muthukumaran, S. Nachimuthu   +2 more
semanticscholar   +1 more source