Results 11 to 20 of about 4,220,889 (382)

Analysis of genomic CFTR DNA [PDF]

Journal of Cystic Fibrosis, 2004
There are numerous methodologies available for the analysis of genomic CFTR DNA. We present here the basic tools to allow a thorough investigation of the CFTR gene, beginning with the identification of potential regulatory regions using DNase I hypersensitive sites, and continuing with methods for the detection of mutations: denaturing High Performance
Marie Pierre Audrézet, Carlos M. Farinha, Javier Sanz, Margarida D. Amaral, Martin Schwarz, Bernhard Steiner, Cédric Le Maréchal, Sabina Gallati, Nathalie Mouchel, Ann Harris, Claude Férec   +10 more
openaire   +3 more sources

Analysis of DNA isolated from different oil sources: problems and solution [PDF]

Iranian Journal of Veterinary Medicine, 2017
Background: One of the major aspects of traceability in food authenticity assessment is to explore practical methods to find the origin of food. Objective: The aim of the present study was to find a DNA based method for authentication and traceability ...
Parviz shayan, Ghazal Nemati, Abolfazl Kamkar, Brigitte Eckert, Afshin Akhondzadeh Basti, Negin Nouri, Iraj Ashrafi Tamai1   +6 more
doaj   +1 more source

Novel compound heterozygous stop‐gain mutations of LRBA in a Vietnamese patient with Common Variable Immune Deficiency

Molecular Genetics & Genomic Medicine, 2020
Background Lipopolysaccharide‐responsive and beige‐like anchor (LRBA) deficiency is a rare autosomal recessive common variable immunodeficiency (CVID), affecting 1:25,000–1:50,000 people worldwide.
Anh N. L. Phan, Thuy T. T. Pham, Nghia Huynh, Tuan M. Nguyen, Cuc T. T. Cao, Duong T. Nguyen, Duc T. Le, Chi‐Bao Bui   +7 more
doaj   +1 more source

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Nature, 2016
Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target locus as the first step to gene correction. Although most genetic diseases arise from point mutations, current approaches to point mutation correction are ...
Alexis C. Komor, Yongjoo B. Kim, M. Packer, John A Zuris, David R. Liu   +4 more
semanticscholar   +1 more source

DNA Checkpoint and Repair Factors Are Nuclear Sensors for Intracellular Organelle Stresses-Inflammations and Cancers Can Have High Genomic Risks. [PDF]

, 2018
Under inflammatory conditions, inflammatory cells release reactive oxygen species (ROS) and reactive nitrogen species (RNS) which cause DNA damage. If not appropriately repaired, DNA damage leads to gene mutations and genomic instability.
Chen, Hongping, Choi, Eric T., Cueto, Ramon, Drosatos, Konstantinos, Fu, Hangfei, Nanayakkara, Gayani K, Park, Joon Y., Qin, Xuebin, Shao, Lijian, Shao, Ying, Sheng, Haitao, Sun, Jianxin, Sun, Yu, Wang, Hong, Wang, Luqiao, Wu, Na, Yang, Qian, Yang, William Y, Yang, Wuping, Yang, Xiaofeng, Zeng, Huihong, Zhu, Qingxian   +21 more
core   +2 more sources

Metal-Binding Antimicrobial Peptide SIF4 Kills Escherichia coli by Targeting Cytoplasmic Biomacromolecules without Cytoplasmic Membrane Damage: A Mechanistic Study [PDF]

Shipin Kexue, 2023
To explore how metal-binding antimicrobial peptide SIF4 kills foodborne Escherichia coli by targeting nucleic acid and protein in the cytoplasmic membrane without cytoplasmic membrane damage, the effect of SIF4 on intracellular nucleic acid biosynthesis ...
LI Yuzhen, XIAO Huaiqiu, LIU Miao, WANG Lin, ZENG Mengqi, ZHAO Mouming
doaj   +1 more source

Prediction of complete gene structures in human genomic DNA.

Journal of Molecular Biology, 1997
We introduce a general probabilistic model of the gene structure of human genomic sequences which incorporates descriptions of the basic transcriptional, translational and splicing signals, as well as length distributions and compositional features of ...
C. Burge, S. Karlin
semanticscholar   +1 more source

Not seeing the genomes for the DNA [PDF]

Briefings in Functional Genomics, 2012
This Short Communication highlights the diversity of 'secondary' genome data (like mitochondrial and plastid genomes) that can be gleaned from next-generation sequencing projects, and encourages researchers to be mindful that these data are often as informative and useful as the 'primary' genome data.
openaire   +3 more sources

An investigation into inter- and intragenomic variations of graphic genomic signatures [PDF]

, 2015
We provide, on an extensive dataset and using several different distances, confirmation of the hypothesis that CGR patterns are preserved along a genomic DNA sequence, and are different for DNA sequences originating from genomes of different species ...
Karamichalis, Rallis, Kari, Lila, Konstantinidis, Stavros, Kopecki, Steffen   +3 more
core   +3 more sources

MicroRNAs down-regulate homologous recombination in the G1 phase of cycling cells to maintain genomic stability

eLife, 2014
Homologous recombination (HR)-mediated repair of DNA double-strand break (DSB)s is restricted to the post-replicative phases of the cell cycle. Initiation of HR in the G1 phase blocks non-homologous end joining (NHEJ) impairing DSB repair.
Young Eun Choi, Yunfeng Pan, Eunmi Park, Panagiotis Konstantinopoulos, Subhajyoti De, Alan D'Andrea, Dipanjan Chowdhury   +6 more
doaj   +1 more source