Results 41 to 50 of about 1,136,601 (292)

ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON [PDF]

Journal of Central European Agriculture, 2002
A melon genomic library was used to isolate and characterize a clone of genomic DNA coding for ascorbate oxidase (AO) which is considered a key enzyme in the biodegradation of ascorbic acid (AA).
Evangelos ALATSATIANOS, Marin ARDELEAN, Constantin BOTEZ, Mircea SAVATTI, Radu SESTRAŞ, Mirela CORDEA   +5 more
doaj  

The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls

Scientific Papers Animal Science and Biotechnologies, 2023
The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNP genotyping. The objective of this study was to apply the high resolution melting analysis for detection of SNP polymorphism CAPN530 of bovine ...
Michal Gábor, Anna Trakovická, Martina Miluchová, Nikola Kleknerová   +3 more
doaj  

Isolation and Molecular Identification of Bacterial Strains to Study Biofilm Formation and Heavy Metals Resistance in Saudi Arabia

Journal of Pure and Applied Microbiology, 2019
Heavy metals found in nature and the excessive accumulations of heavy metals have an impact on humans and animals. Different effects resulted from the toxicity of heavy metal, that damage the functioning of different organs: brain, lungs, kidney and ...
Rania M. Makki, Ola I. M. El-Hamshary, Zainah M. Almarhabi   +2 more
doaj   +1 more source

Pre-diagnostic leukocyte genomic DNA methylation and the risk of colorectal cancer in women. [PDF]

PLoS ONE, 2013
Abnormal one-carbon metabolism may lead to general genomic (global) hypomethylation, which may predispose an individual to the development of colorectal neoplasia.We evaluated the association between pre-diagnostic leukocyte genomic DNA methylation level
Hongmei Nan, Edward L Giovannucci, Kana Wu, Jacob Selhub, Ligi Paul, Bernard Rosner, Charles S Fuchs, Eunyoung Cho   +7 more
doaj   +1 more source

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer

Cell Reports, 2016
High-grade serous ovarian carcinomas (HGSOCs) with BRCA1/2 mutations exhibit improved outcome and sensitivity to double-strand DNA break (DSB)-inducing agents (i.e., platinum and poly(ADP-ribose) polymerase inhibitors [PARPis]) due to an underlying ...
Young Eun Choi, Khyati Meghani, Marie-Eve Brault, Lucas Leclerc, Yizhou J. He, Tovah A. Day, Kevin M. Elias, Ronny Drapkin, David M. Weinstock, Fanny Dao, Karin K. Shih, Ursula Matulonis, Douglas A. Levine, Panagiotis A. Konstantinopoulos, Dipanjan Chowdhury   +14 more
doaj   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Specific Primer Design for Detection and Quantification of Entomopathogenic Fungi Metarhizium anisopliae using Quantitative PCR (qPCR) in Soil and Cocoon Samples

AGRIVITA Journal of Agricultural Science
The information on the abundance and dynamics of entomopathogenic fungus Metarhizium anisopliae on the soil is limited by the existence of insect hosts. In this study, to detect and quantify specifically for M.
Syaiful Amri Saragih, Shuhei Takemoto, Naoto Kamata   +2 more
doaj   +1 more source

A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules

Cell Reports, 2013
DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a ...
Andres J. Lopez-Contreras, Isabel Ruppen, Maria Nieto-Soler, Matilde Murga, Sara Rodriguez-Acebes, Silvia Remeseiro, Sara Rodrigo-Perez, Ana M. Rojas, Juan Mendez, Javier Muñoz, Oscar Fernandez-Capetillo   +10 more
doaj   +1 more source

Genome-Scale Dna Methylation Analysis [PDF]

Epigenomics, 2010
The haploid human genome contains approximately 29 million CpGs that exist in a methylated, hydroxymethylated or unmethylated state, collectively referred to as the DNA methylome. The methylation status of cytosines in CpGs and occasionally in non-CpG cytosines influences protein–DNA interactions, gene expression, and chromatin structure and stability.
Shaun D, Fouse, Raman O, Nagarajan, Joseph F, Costello   +2 more
openaire   +2 more sources