Results 71 to 80 of about 4,220,889 (382)

Microarray-based ultra-high resolution discovery of genomic deletion mutations [PDF]

, 2014
BACKGROUND: Oligonucleotide microarray-based comparative genomic hybridization (CGH) offers an attractive possible route for the rapid and cost-effective genome-wide discovery of deletion mutations.
Baban, D., Belfield, E., Brown, C., Gan, X., Harberd, N., Jiang, C., Mithani, A., Mott, R., Ragoussis, J.   +8 more
core   +3 more sources

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Targeting Dna Methylation to the Genome

BioTechniques, 2014
Proving direct relationships between DNA alterations and phenotypes is challenging. For epigenetics researchers, linking DNA methylation with human disease is no exception. But Patrick Lo looks at how two researchers are developing new methods to try to trace the road from DNA methylation to human biology.
openaire   +4 more sources

Co-transcriptional R-loops are the main cause of estrogen-induced DNA damage. [PDF]

, 2016
The hormone estrogen (E2) binds the estrogen receptor to promote transcription of E2-responsive genes in the breast and other tissues. E2 also has links to genomic instability, and elevated E2 levels are tied to breast cancer.
Bocek, Michael, Chédin, Frédéric, Cimprich, Karlene A, Crossley, Madzia P, Sanz, Lionel A, Sollier, Julie, Stork, Caroline Townsend, Swigut, Tomek   +7 more
core   +3 more sources

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

An efficient DNA Extraction Method from Ruditapes philippinarum larvae and Its Application in Genetic Analysis

Progress in Fishery Sciences
Ruditapes philippinarum—a marine bivalve mollusc known for its adaptability across a range of temperatures, salinities, and habitats—thrives primarily in tidal flats and shallow marine areas. It is an ideal candidate for high-density cultivation in tidal
Lei ZHANG, Xinghong XU, Zhihong LIU, Biao WU, Liqing ZHOU, Zhuanzhuan LI, Peizhen MA, Xiujun SUN   +7 more
doaj   +1 more source

DNA methylation profiling of genomic DNA isolated from urine in diabetic chronic kidney disease: A pilot study. [PDF]

PLoS ONE, 2018
To characterise the genomic DNA (gDNA) yield from urine and quality of derived methylation data generated from the widely used Illuminia Infinium MethylationEPIC (HM850K) platform and compare this with buffy coat samples.DNA methylation is the most ...
Ashani Lecamwasam, Alexandra Sexton-Oates, Jake Carmody, Elif I Ekinci, Karen M Dwyer, Richard Saffery   +5 more
doaj   +1 more source

Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species

Plant Methods, 2014
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA ...
A. Healey, A. Furtado, Tal Cooper, R. Henry   +3 more
semanticscholar   +1 more source

Measuring DNA mechanics on the genome scale [PDF]

Nature, 2020
AbstractMechanical deformations of DNA such as bending are ubiquitous and implicated in diverse cellular functions1. However, the lack of high-throughput tools to directly measure the mechanical properties of DNA limits our understanding of whether and how DNA sequences modulate DNA mechanics and associated chromatin transactions genome-wide.
Thuy T.M. Ngo, Cynthia Wolberger, Aakash Basu, Aakash Basu, Anand Ranjan, Miroslav Hejna, Taekjip Ha, Sebastian Eustermann, Zan Qureshi, Michael T. Morgan, Dmitriy G. Bobrovnikov, Jun S. Song, Basilio Cieza, Tunc Kayikcioglu, Tunc Kayikcioglu, Karl-Peter Hopfner, H. Tomas Rube   +16 more
openaire   +5 more sources

Somatic mutation load of estrogen receptor-positive breast tumors predicts overall survival: an analysis of genome sequence data. [PDF]

, 2014
Breast cancer is one of the most commonly diagnosed cancers in women. While there are several effective therapies for breast cancer and important single gene prognostic/predictive markers, more than 40,000 women die from this disease every year.
Bainbridge, Matthew N, Brown, Powel H, Haricharan, Svasti, Scheet, Paul   +3 more
core   +1 more source