Results 81 to 90 of about 4,220,889 (382)

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON [PDF]

Journal of Central European Agriculture, 2002
A melon genomic library was used to isolate and characterize a clone of genomic DNA coding for ascorbate oxidase (AO) which is considered a key enzyme in the biodegradation of ascorbic acid (AA).
Evangelos ALATSATIANOS, Marin ARDELEAN, Constantin BOTEZ, Mircea SAVATTI, Radu SESTRAŞ, Mirela CORDEA   +5 more
doaj  

Pre-diagnostic leukocyte genomic DNA methylation and the risk of colorectal cancer in women. [PDF]

PLoS ONE, 2013
Abnormal one-carbon metabolism may lead to general genomic (global) hypomethylation, which may predispose an individual to the development of colorectal neoplasia.We evaluated the association between pre-diagnostic leukocyte genomic DNA methylation level
Hongmei Nan, Edward L Giovannucci, Kana Wu, Jacob Selhub, Ligi Paul, Bernard Rosner, Charles S Fuchs, Eunyoung Cho   +7 more
doaj   +1 more source

A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods

, 2015
We report a rapid and efficient method of genomic DNA extraction from filamentous fungi with high-throughput potential. The method involves disruption of fungal cell by bead beating in a homogenizer, followed by RNase treatment, phenol: chloroform ...
Sutar Aamir S, Baghela
semanticscholar   +1 more source

Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique [PDF]

, 2010
Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree [Tetraclinis articulata (Vahl) Masters] is often limited by the
Abourouh, Mohamed, Bakkali Yakhlefb, Salaheddine, Guenoun, Imane, Hamamouch, Noureddine, kerdouh, Benaîssa   +4 more
core   +2 more sources

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

The Use of High Resolution Melting Analysis for Detection of the CAPN530 Polymorphism in Slovak Simmental Bulls

Scientific Papers Animal Science and Biotechnologies, 2023
The high-resolution melting analysis is a cost-effective and simple post-PCR technique that can be used for SNP genotyping. The objective of this study was to apply the high resolution melting analysis for detection of SNP polymorphism CAPN530 of bovine ...
Michal Gábor, Anna Trakovická, Martina Miluchová, Nikola Kleknerová   +3 more
doaj  

Surface mucous as a source of genomic DNA from Atlantic billfishes (Istiophoridae) and swordfish (Xiphiidae) [PDF]

, 2009
Procedures for sampling genomic DNA from live billfishes involve manual restraint and tissue excision that can be difficult to carry out and may produce stresses that affect fish survival.
Bernard, Andrea M., Faugue, Ronald M., Hoolihan, John P., Horn, Rebekah L., Perez, Nerida F., Schultz, Duane R., Snodgrass, Derke   +6 more
core   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

The nicotinamide hypothesis revisited—plant defense signaling integrating PARP, nicotinamide, nicotinic acid, epigenetics, and glutathione

FEBS Letters, EarlyView.
Nicotinamide (NIC) and nicotinic acid (NIA) are proposed as stress signaling compounds in plants. Oxidative stress may lead to single strand breaks (SSB) in DNA, which activate poly(ADP‐ribose) polymerase (PARP). NIC and NIA are then formed from NAD. NIC and NIA can promote epigenetic changes leading to the expression of defense genes specific for the ...
Torkel Berglund, Anna B. Ohlsson
wiley   +1 more source