Results 181 to 190 of about 22,130 (225)

Isolation and Characterization of Random Citrate Synthase Variants That Increase Acetate Generation by Escherichia coli

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin   +3 more
wiley   +1 more source

Genetic Architecture of Grain Protein Content in Hulless Barley: Insights from Multi-Environment GWAS. [PDF]

open access: yesPlants (Basel)
Memon S   +15 more
europepmc   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari   +11 more
wiley   +1 more source

Kin discrimination in plants: overview and implications for population and community ecology

open access: yesBiological Reviews, EarlyView.
ABSTRACT Following the discovery of identity discrimination, particularly self and kin discrimination amongst plant competitors, research on interplant interactions has advanced significantly within plant physiology and evolutionary ecology. This review synthesizes current knowledge on how both self and kin discrimination influence plant growth ...
Akira Yamawo
wiley   +1 more source

Mild Hydrothermal Stabilization: An Innovative Technology for Improving Whole‐Grain Rice Quality in West Africa and Elsewhere

open access: yesCereal Chemistry, EarlyView.
ABSTRACT Background and Objectives Whole‐grain (brown) rice has superior nutritional quality but is prone to rancidity, primarily caused by endogenous lipases and oxidases. This study evaluated mild hydrothermal stabilization (MHS), comprising grain surface wetting, followed by short‐duration atmospheric‐pressure steaming, applied to two West African ...
Sali A. Ndindeng   +5 more
wiley   +1 more source

Learning sparse mixture‐of‐experts generalized linear models in ultrahigh dimensions

open access: yesCanadian Journal of Statistics, EarlyView.
Abstract Motivated by the challenges of heterogeneity and high dimensionality in the analysis of modern data, we investigate continuous regularization methods for learning sparse mixture‐of‐experts generalized linear models (MoE‐GLM). Although there are foundational results about regularized estimators in a broad class of regression models including ...
Pengqi Liu, Abbas Khalili
wiley   +1 more source

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