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The Use of Immunological Techniques to Detect Cells of Rare Genotype
1982An increase in the mutation rate in germinal cells may result in an increase in the number of genetically defective persons in the next and future generations, and mutations in somatic cells may be the possible cause of such diseases as cancer(1–3) and of advanced aging.(4) These health effects result in decreased fitness and life-span of and emotional
Aftab A. Ansari, H. V. Mailing
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Improvement on PCR-CTPP: a SNP genotyping approach based on mismatch technique
Hereditas (Beijing), 2011To explore the technique principle of PCR with confronting two-pair primers (PCR-CTPP) and improve the accuracy of SNP genotyping by taking the 1298 locus of human gene MTHFR as an example, the reliability between conventional PCR-CTPP and improved PCR-CTPP was compared using reconstructed PCR-CTPP detecting system in terms of designing appropriate ...
Yu-Xia Yun +8 more
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Evaluation of fingerprinting techniques to assess genotype variation among Zygosaccharomyces strains
Food Microbiology, 2018Molecular typing techniques are key tools in surveillance of food spoilage yeasts, in investigations on intra-species population diversity, and in tracing selected starters during fermentation. Unlike previous works on strain typing of Zygosaccharomyces spoilage species, here Zygosaccharomyces mellis and the Zygosaccharoymces rouxii complex yeasts ...
Dakal TC, Solieri L, Giudici P.
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Clinica Chimica Acta, 1982
Abstract Semi-micro techniques are presented for the genotyping of galactokinase and galactose-1-phosphate uridyltransferase. Radioactive assays are used to quantitate the levels of enzyme activity, while agarose gel electrophoresis is used to type the variants of galactose-1-phosphate uridyltransferase.
Jo Ellen Sheets Lee, Won G. Ng
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Abstract Semi-micro techniques are presented for the genotyping of galactokinase and galactose-1-phosphate uridyltransferase. Radioactive assays are used to quantitate the levels of enzyme activity, while agarose gel electrophoresis is used to type the variants of galactose-1-phosphate uridyltransferase.
Jo Ellen Sheets Lee, Won G. Ng
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PCR-CTPP: a new genotyping technique in the era of genetic epidemiology
Expert Review of Molecular Diagnostics, 2001A new PCR method, PCR-CTPP (polymerase chain reaction with confronting two-pair primers) was invented to genotype a relatively large number of samples in a cost-effective and time-saving manner. In this method, allele-specific DNA products are amplified by means of applying appropriately designed two-pair primers (four primers) into an ordinary PCR ...
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Orogenital transmission of Neisseria meningitidis serogroup C confirmed by genotyping techniques
European Journal of Clinical Microbiology & Infectious Diseases, 2004Urethritis caused by Neisseria meningitidis in heterosexual patients is presumed to occur via orogenital contact, but confirmation has not been possible in most cases. Presented here is a case of urethritis caused by N. meningitidis, serogroup C, and the isolation of the same microorganism from the nasopharynx and endocervix of the patient's sexual ...
I. Martínez +6 more
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Molecular Techniques for Genotyping
2020Genotyping is a process of determining the genetic constituent/genetic makeup “genotype” of an organism by examining the individual DNA sequence and comparing to a reference or other individual sequence. It helps the researchers to explore the genetic constitution, genetic linkages or variations like Single Nucleotide Polymorphisms (SNP) or multi ...
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GENOTYPE RESPONSE TO IN VITRO GYNOGENESIS TECHNIQUE IN GERBERA JAMESONII
Acta Horticulturae, 1990A. Tosca +4 more
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A SCREENING TECHNIQUE FOR LYCHEE GENOTYPES ADAPTED TO WARM CLIMATES
Acta Horticulturae, 1986B.F. Paxton, C.M. Menzel
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