SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Information Extraction of Doctoral Theses Using Two Different Large Language Models vs Health Services Researchers: Development and Usability Study. [PDF]
Cittadino J +8 more
europepmc +1 more source
Comparing evidence use in parliaments: the interplay of beliefs, traditions, and practices in the UK and Germany [PDF]
Marc Geddes
openalex +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
Longitudinal evaluation of RADUCATION: a digital learning environment for the radiology residency structured to a competency-based curriculum by the German Young Radiology Forum. [PDF]
Molwitz I +11 more
europepmc +1 more source
Review Of Angst Und Geschlechterdifferenzen: Ingeborg Bachmanns \u27Todesarten\u27-Projekt In Kontexten Der Gegenwarts By C. Kanz [PDF]
Simon, Sunka
core +1 more source
Climate Change and Urban Resilience in Smart Cities: Adaptation and Mitigation Strategies in Brazil and Germany [PDF]
Roberto Schoproni Bichueti +5 more
openalex +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Application of a preclinical 18-gene classifier to patients with locally advanced HNSCC. [PDF]
Löck S +19 more
europepmc +1 more source

