Results 121 to 130 of about 494 (152)
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GFLV replication in electroporated grapevine protoplasts
Plant Science, 2000Grapevine fanleaf virus (GFLV), responsible for the economically important court-noué disease, is exclusively transmitted to its natural host in the vineyards through Xiphinema nematodes. We have developed direct inoculation of GFLV into grapevine through protoplast electroporation.
, Valat +7 more
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Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine
European Journal of Plant Pathology, 2015Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we
Anastazija Jež Krebelj +3 more
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Molecular characterization of grapevine plants transformed with GFLV resistance genes: I
Plant Cell Reports, 2005The Grapevine FanLeaf Virus-Coat Protein (GFLV CP) gene was inserted through Agrobacterium-mediated transformation in Vitis vinifera "Nebbiolo", "Lumassina" and "Blaufränkisch". Two plasmids were used: pGA-CP+ (full-length GFLV CP gene with an introduced start codon) and pGA-AS (same gene in antisense orientation).
Giorgio Gambino +4 more
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O‘ZBЕKISTONDA IQLIMLASHTIRILGAN TOK O‘SIMLIGINING FANLIF (GFLV) VIRUSINI DIAGNOSTIKA QILISH
2016-yil, 1-son (95) ANIQ VA TABIIY FANLAR SERIYASI, 2023Ushbu maqolada O‘zbekistonda uzumchilik rivojlanishi bilan bir qatorda uzumning virusli kasalliklari keng tarqalganligi bo’yicha ma’lumotlar keltirilgan. Sirdaryo viloyatidagi uzumzorlarda fanlif virus (GFLV) belgilarini o‘rganish maqsadida monitoring o‘tkazildi.
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Molecular characterization of grapevine plants transformed with GFLV resistance genes: II
Plant Cell Reports, 2006A collection of 127 putatively transgenic individuals of Vitis vinifera cv. Russalka was characterized by PCR and Southern hybridization. Six different constructs containing the neomycin phosphotransferase (nptII) marker gene and sequences of the Grapevine Fanleaf Virus Coat Protein (GFLV CP) gene including non-translatable and truncated forms were ...
Fatemeh Maghuly +8 more
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Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China
Archives of Virology, 2015To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38% tested positive for GFLV by DAS-ELISA, and 26.8% tested positive by RT-PCR and nested RT-PCR.
Jun, Zhou +5 more
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High efficiency regeneration of grapevine plants transformed with the GFLV coat protein gene
Plant Science, 1995Abstract Genetically transformed grapevines were obtained through co-cultivation of embryogenic cell suspensions with an engineered A. tumefaciens strain. Two economically important rootstocks, 41B and SO4, as well as a well-known grapevine vinifera variety, Chardonnay were regenerated.
Mauro, M.C. +7 more
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Plant Cell, Tissue and Organ Culture (PCTOC), 2009
Grapevine Fanleaf virus (GFLV) is one of the most damaging and widespread nepovirus in grapevine, causing the fanleaf disease. Here, we report the development of inverted repeat (IR) constructs, consisting of GFLV-derived sequences, for genetic transformation of grapevine to induce GFLV-specific silencing.
Rahma Jardak-Jamoussi +7 more
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Grapevine Fanleaf virus (GFLV) is one of the most damaging and widespread nepovirus in grapevine, causing the fanleaf disease. Here, we report the development of inverted repeat (IR) constructs, consisting of GFLV-derived sequences, for genetic transformation of grapevine to induce GFLV-specific silencing.
Rahma Jardak-Jamoussi +7 more
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Chinese journal of biotechnology, 2000
The cDNA of the GFLV CP gene amplified from clone pGAB5 by PCR was labeled by photosensitive biotin. The probe sensitivity is 5 pg, and the detectable amount of unlabeled GFLV cDNA was 10 pg by Dot-blot hybridization when the biotin-labeled cDNA was used as a probe.
X, Cao +4 more
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The cDNA of the GFLV CP gene amplified from clone pGAB5 by PCR was labeled by photosensitive biotin. The probe sensitivity is 5 pg, and the detectable amount of unlabeled GFLV cDNA was 10 pg by Dot-blot hybridization when the biotin-labeled cDNA was used as a probe.
X, Cao +4 more
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Correlating ELISA values with growth and yield components of GFLV infected grapevines
2015VITIS - Journal of Grapevine Research, Vol. 34 No.
Frantz, E. J., Walker, M. A.
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