Results 41 to 50 of about 494 (152)
Cloning and sequence analysis of P38 gene of Grapevine fanleaf virus Hangzhou isolated
To confirm the characteristics and function of P38 in the viral particles movement, the cDNA fragment of P38 gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA2 sequence of GFLV-F13 and ...
LI Hong-ye, CHEN Li-geng, ZHOU Xue-ping
doaj +1 more source
Abstract The Panel on Plant Health performed a listing of non‐EU viruses and viroids (reported hereinafter as viruses) of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L. A systematic literature review identified 197 viruses infecting one or more of the host genera under consideration. Viruses were allocated
EFSA Panel on Plant Health (PLH) +26 more
wiley +1 more source
Summary To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need to be developed to increase ...
Anna Bastet +12 more
wiley +1 more source
Nanobody‐mediated resistance to Grapevine fanleaf virus in plants
Summary Since their discovery, single‐domain antigen‐binding fragments of camelid‐derived heavy‐chain‐only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited.
Caroline Hemmer +20 more
wiley +1 more source
Tubule-guided cell-to-cell movement of a plant virus requires class XI myosin motors. [PDF]
Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral movement proteins (MP) modify PD to allow passage of the virus particles or nucleoproteins.
Khalid Amari +4 more
doaj +1 more source
Summary For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus‐resistant transgenic plants based on the pathogen‐derived resistance strategy has been a success story for over three decades.
Jean‐Michel Hily +16 more
wiley +1 more source
Elimination of Grapevine fanleaf virus in grapevine by in vivo and in vitro thermotherapy
In this paper, results of the in vitro treatment are compared with those of in vivo therapy (i.e. treatment of plants in a peat substrate) when eliminating GFLV (Grapevine fanleaf virus) from three grapevine rootstocks.
B. Křižan +4 more
doaj +1 more source
Solubilization and Refolding of Inclusion Body of Grapevine fanleaf virus-coat Protein Produced in E. coli [PDF]
Objective High level expression of recombinant protein in Escherichia coli often results in aggregation of the expressed protein molecules into inclusion bodies. Cysteines in the protein contribute to this process.
Razieh Yazdani +3 more
doaj +1 more source
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein.
Davoud Koolivand +3 more
doaj +1 more source
Next‐generation methods for early disease detection in crops
Innovative alternative diagnostics allow easier and quicker plant disease detection than traditional methods. These latter methodological approaches require specialized personnel and they usually are more expensive than innovative methods based on field detection with portable instruments which are accessible to non‐specialized personnel.
Daniela Trippa +10 more
wiley +1 more source

