Results 41 to 50 of about 494 (152)

Cloning and sequence analysis of P38 gene of Grapevine fanleaf virus Hangzhou isolated

open access: yes浙江大学学报. 农业与生命科学版, 2003
To confirm the characteristics and function of P38 in the viral particles movement, the cDNA fragment of P38 gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA2 sequence of GFLV-F13 and ...
LI Hong-ye, CHEN Li-geng, ZHOU Xue-ping
doaj   +1 more source

List of non‐EU viruses and viroids of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L.

open access: yesEFSA Journal, Volume 17, Issue 9, September 2019., 2019
Abstract The Panel on Plant Health performed a listing of non‐EU viruses and viroids (reported hereinafter as viruses) of Cydonia Mill., Fragaria L., Malus Mill., Prunus L., Pyrus L., Ribes L., Rubus L. and Vitis L. A systematic literature review identified 197 viruses infecting one or more of the host genera under consideration. Viruses were allocated
EFSA Panel on Plant Health (PLH)   +26 more
wiley   +1 more source

Trans‐species synthetic gene design allows resistance pyramiding and broad‐spectrum engineering of virus resistance in plants

open access: yesPlant Biotechnology Journal, Volume 16, Issue 9, Page 1569-1581, September 2018., 2018
Summary To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need to be developed to increase ...
Anna Bastet   +12 more
wiley   +1 more source

Nanobody‐mediated resistance to Grapevine fanleaf virus in plants

open access: yesPlant Biotechnology Journal, Volume 16, Issue 2, Page 660-671, February 2018., 2018
Summary Since their discovery, single‐domain antigen‐binding fragments of camelid‐derived heavy‐chain‐only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited.
Caroline Hemmer   +20 more
wiley   +1 more source

Tubule-guided cell-to-cell movement of a plant virus requires class XI myosin motors. [PDF]

open access: yesPLoS Pathogens, 2011
Cell-to-cell movement of plant viruses occurs via plasmodesmata (PD), organelles that evolved to facilitate intercellular communications. Viral movement proteins (MP) modify PD to allow passage of the virus particles or nucleoproteins.
Khalid Amari   +4 more
doaj   +1 more source

Metagenomic‐based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome

open access: yesPlant Biotechnology Journal, Volume 16, Issue 1, Page 208-220, January 2018., 2018
Summary For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus‐resistant transgenic plants based on the pathogen‐derived resistance strategy has been a success story for over three decades.
Jean‐Michel Hily   +16 more
wiley   +1 more source

Elimination of Grapevine fanleaf virus in grapevine by in vivo and in vitro thermotherapy

open access: yesHorticultural Science, 2009
In this paper, results of the in vitro treatment are compared with those of in vivo therapy (i.e. treatment of plants in a peat substrate) when eliminating GFLV (Grapevine fanleaf virus) from three grapevine rootstocks.
B. Křižan   +4 more
doaj   +1 more source

Solubilization and Refolding of Inclusion Body of Grapevine fanleaf virus-coat Protein Produced in E. coli [PDF]

open access: yesمجله بیوتکنولوژی کشاورزی, 2019
Objective High level expression of recombinant protein in Escherichia coli often results in aggregation of the expressed protein molecules into inclusion bodies. Cysteines in the protein contribute to this process.
Razieh Yazdani   +3 more
doaj   +1 more source

Production of Polyclonal Antibody against Grapevine fanleaf virus Movement Protein Expressed in Escherichia coli

open access: yesThe Plant Pathology Journal, 2016
The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein.
Davoud Koolivand   +3 more
doaj   +1 more source

Next‐generation methods for early disease detection in crops

open access: yesPest Management Science, Volume 80, Issue 2, Page 245-261, February 2024.
Innovative alternative diagnostics allow easier and quicker plant disease detection than traditional methods. These latter methodological approaches require specialized personnel and they usually are more expensive than innovative methods based on field detection with portable instruments which are accessible to non‐specialized personnel.
Daniela Trippa   +10 more
wiley   +1 more source

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