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Hippocampus, 2008
AbstractGranule cells of the mouse fascia dentata are widely used in studies on neuronal development and plasticity. In contrast to the rat, however, high‐resolution morphometric data on these cells are scarce. Thus, we have analyzed granule cells in the fascia dentata of the adult Thy1‐GFP mouse (C57BL/6 background). In this mouse line, single neurons
Vukšić, Mario +7 more
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AbstractGranule cells of the mouse fascia dentata are widely used in studies on neuronal development and plasticity. In contrast to the rat, however, high‐resolution morphometric data on these cells are scarce. Thus, we have analyzed granule cells in the fascia dentata of the adult Thy1‐GFP mouse (C57BL/6 background). In this mouse line, single neurons
Vukšić, Mario +7 more
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Methods for Expressing and Analyzing GFP-Tubulin and GFP-Microtubule-Associated Proteins
Cold Spring Harbor Protocols, 2010INTRODUCTIONImportant advances in our understanding of the organization and dynamics of the cytoskeleton have been made by direct observations of fluorescently tagged cytoskeletal proteins in living cells. In early experiments, the cytoskeletal protein of interest was purified, covalently modified with a fluorescent dye, and microinjected into living ...
Holly V, Goodson +2 more
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Chemical & Engineering News Archive, 2009
CHEMISTS HAVE USED ultrafast Raman spectroscopy to map the structural evolution of the green fluorescent protein (GFP) chromophore during proton transfer. The findings not only help explain the workings of GFP but could also lead to a better fundamental understanding of proton transfer, which drives key biological processes. “Since the discovery in our
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CHEMISTS HAVE USED ultrafast Raman spectroscopy to map the structural evolution of the green fluorescent protein (GFP) chromophore during proton transfer. The findings not only help explain the workings of GFP but could also lead to a better fundamental understanding of proton transfer, which drives key biological processes. “Since the discovery in our
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2011
?????????????????????????????? ???????????????????????????? ???????????????????????????? ?????????????? Ac-CMV-GFP, Ac-M-GFP ?? Ac-IFN-GFP ?? ???????????????????? ?????????????????????????? ???????????????????? (???????????????? CMV ?? ?????????????? CAG), ???????????????????????? ???????????????????? ???????????????????????? ???????? eGfp ?? ???????? ?
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?????????????????????????????? ???????????????????????????? ???????????????????????????? ?????????????? Ac-CMV-GFP, Ac-M-GFP ?? Ac-IFN-GFP ?? ???????????????????? ?????????????????????????? ???????????????????? (???????????????? CMV ?? ?????????????? CAG), ???????????????????????? ???????????????????? ???????????????????????? ???????? eGfp ?? ???????? ?
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Nature Methods, 2005
An oligomerization-dependent system comprising two fragments of green fluorescent protein (GFP) fused to engineered zinc fingers could be a means to detect virtually any double-stranded DNA sequence.
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An oligomerization-dependent system comprising two fragments of green fluorescent protein (GFP) fused to engineered zinc fingers could be a means to detect virtually any double-stranded DNA sequence.
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The green fluorescent protein (GFP) has enabled researchers to visualize a wide range of cellular processes, from protein expression to metastasis. However, its size (27 kDa) can disrupt localization and association of GFP-tagged proteins. Here, we aim to address this limitation by designing a miniature GFP (<20 kDa) that conserves its chromophore ...
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2021
The results of Agrobacterium-mediated transformation of different flax cultivars by plasmid carrying chimeric GFP-TUA6 gene are presented in this study. Transgenic nature of obtained lines was confirmed by PCR analysis. It was shown that GFP labeled tubulin is capable to co-polymerize with endogenous cell tubulin and to participate in cortical ...
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The results of Agrobacterium-mediated transformation of different flax cultivars by plasmid carrying chimeric GFP-TUA6 gene are presented in this study. Transgenic nature of obtained lines was confirmed by PCR analysis. It was shown that GFP labeled tubulin is capable to co-polymerize with endogenous cell tubulin and to participate in cortical ...
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