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Reconstitution and Anchoring of Cytoskeleton inside Giant Unilamellar Vesicles
ChemBioChem, 2008AbstractAmong the requirements for all life forms is the ability to self‐replicate. In eukaryotic cellular systems, this division is achieved through cytokinesis, and is facilitated by the (re)arrangement and interaction of cytoskeletal proteins with lipids and other proteins localized to the plasma membrane. A fascinating challenge of modern synthetic
Nicoletta Kahya+2 more
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Deformation Modes of Giant Unilamellar Vesicles Encapsulating Biopolymers
ACS Synthetic Biology, 2018The shapes of giant unilamellar vesicles (GUVs) enclosing polymer molecules at relatively high concentration, used as a model cytoplasm, significantly differ from those containing only small molecules. Here, we investigated the effects of the molecular weights and concentrations of polymers such as polyethylene glycol (PEG), bovine serum albumin (BSA),
Taiji Okano+3 more
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Block copolymers in giant unilamellar vesicles with proteins or with phospholipids
Faraday Discussions, 2013Biocompatible, highly water-soluble, nonionic, amphiphilic block copolymers having different hydrophobic blocks and architectures, but similar molecular size and chemical nature of the hydrophilic blocks, were investigated to check for their ability to form hybrid giant unilamellar vesicles with proteins, and for their interactions with giant ...
Elkin Amado+4 more
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Electrofusion of Mammalian Cells and Giant Unilamellar Vesicles
1989Cell electrofusion is one of the most striking observations linked to the effect of electric fields on viable cells or protoplasts. Since its first applications in the early 1980s, this methodology has been developed in many fields of cell biology and biotechnology. Different applications are reviewed in this book.
Marie-Pierre Rols+2 more
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Electrodeformation, Electroporation, and Electrofusion of Giant Unilamellar Vesicles
2016Karin A. Riske, Rumiana Dimova
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