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Studies on the Mechanism of Collagen Glucosyltransferase Reaction [PDF]
The mechanism of collagen glucosyltransferase reaction was studied with enzyme preparations purified about 2500-5000-fold from extract of homogenate of whole chick embryos. Data obtained in experiments on initial velocity and inhibition kinetics of the reaction were consistent with an ordered mechanism in which the substrates are bound to the enzyme in
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Specific inhibition of glucosyltransferase of Streptococcus mutans
Carbohydrate Research, 1980Clinical dextran, partially oxidized with sodium periodate, acts as a potent inhibitor of the extracellular glucosyltransferases of several cariogenic strains of oral Streptococcus mutans. Preincubation with oxidized dextran resulted in a rapid loss of up to 80% of the ability of the enzyme preparation to synthesize polysaccharide from sucrose, but ...
Eric E. Smith, Masakazu Inoue
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The determination of glucosyltransferase
Biotechnology Letters, 1979A new procedure for the determination of glucosyltransferase (EC 2.4.1.24) activity in amyloglucosidase preparations has been developed. The procedure is based on the rapid separation of glucosyltransferase from amyloglucosidase on carboxymethyl bio-gel A.
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Regulation of Streptococcus gordonii Glucosyltransferase
1997The early dental plaque organism Streptococcus gordonii has a single glucosyltransferase (GTF) enzyme that makes both α1,3- and α1,6-linked glucans from sucrose(5). The glucans made by the parental strain Challis CH1 confer a hard, cohesive, sucrose-promoted phenotype (Spp+) to colonies grown on sucrose agar(3). Spp− variants, which are soft on sucrose
Don B. Clewell, M. Margaret Vickerman
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Cytotoxic Glucosyltransferases of Legionella pneumophila
2013Legionella is a gram-negative bacterium and the causative pathogen of legionellosis-a severe pneumonia in humans. A large number of Legionella effectors interfere with numerous host cell functions, including intracellular vacuole trafficking and maturation, phospholipid metabolism, protein ubiquitination, pro-/anti-apoptotic balances or inflammatory ...
Belyi, Yury+2 more
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The glucosyltransferases of Streptococcus salivarius
Australian Dental Journal, 1994AbstractThis review covers some of the more recent developments in the understanding of the different glucosyltransferases (GTFs) secreted by oral streptococci, particularly those produced by Streptococcus salivarius — a species that has been intensively studied at the Institute of Dental Research in Sydney.
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Glucosyltransferase Inactivation Reduces Dental Caries
Journal of Dental Research, 2001Dental caries has been an intractable disease in spite of intense dental research. The metabolic acids produced by mutans streptococci demineralize the tooth surface and lead to dental caries. The enzyme glucosyltransferase (GTF) produced by mutans streptococci is the key factor in this process.
K.S. Devulapalle, G. Mooser
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Uridine-diphosphoglucose glucosyltransferase in human erythrocytes
Clinica Chimica Acta, 1965Abstract The presence of glucosyltransferase activity in mature human erythrocytes and leukocytes has been established both by demonstration of UDP formation from UDPG, and by incorporation of [14C] glucose from UDP-[14C] glucose into glycogen. The erythrocyte and leukocyte enzymes have similar properties and both require glucose 6-P for activity ...
M. Cornblath+3 more
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Biotransformation into 11α-hydroxyprogesterone glucosides by glucosyltransferase
Steroids, 2019Recently, studies on the steroidal hormone activity in the brain have attracted attention, and the influences of the varied glucosides and their artificial derivatives have been discussed; additionally, it has been suggested that glucosides are the synthetic precursors of glucuronide as a label molecule.
Tokutaro Yamaguchi+4 more
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Glucosyltransferase isozymes in algae
Phytochemistry, 1967Abstract Purified phosphorylase preparations from representative members of three algal Groups were subjected to two-dimensional electrophoresis on polyacrylamide gel. Three groups of closely associated isozymes were found in each preparation. Two isozymes of the conventional phosphorylase type were present in preparations from blue-green and from ...
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