Results 241 to 250 of about 191,408 (290)

Inherited metabolic epilepsies–established diseases, new approaches

open access: yesEpilepsia Open, EarlyView.
Abstract Inherited metabolic epilepsies (IMEs) represent the inherited metabolic disorders (IMDs) in which epilepsy is a prevailing component, often determining other neurodevelopmental outcomes associated with the disorder. The different metabolic pathways affected by individual IMEs are the basis of their rarity and heterogeneity.
Itay Tokatly Latzer, Phillip L. Pearl
wiley   +1 more source

Development of Komagataella phaffii Cell‐Free Protein Synthesis Using Cell From Basic Salt Medium

open access: yesFood Bioengineering, EarlyView.
This process illustrates a three‐step procedure for K. phaffii CFPS setup. First, K. phaffii cells are cultivated in basic salt medium and harvested by centrifugation. Then, cell extract is prepared from cell pellets through ultrasonic disruption, dialysis, centrifugation, and lyophilisation. Finally, K.
Yu Zhang   +4 more
wiley   +1 more source

Multiple Strategies for Recombinant Expression of Human Chymotrypsinogen C in Pichia Pastoris

open access: yesFood Bioengineering, EarlyView.
Human chymotrypsin C (CTRC), a food‐processing protease, was expressed in Pichia pastoris via three strategies: replacing the AOX1 promoter with HpHMD, rDNA locus‐based gene integration, and engineering the pro2M mutant to evade host protease degradation. The pro2M showed 3.3‐fold higher specific activity (54.84 U/mg) and improved secretion relative to
Yi Liu   +5 more
wiley   +1 more source

Glycolytic flux sustains human Th1 identity and effector function via STAT1 glycosylation. [PDF]

open access: yesLife Sci Alliance
Abir AH   +8 more
europepmc   +1 more source

Development, Scope, and Catalyst Selection in Boron-Catalyzed Iterative Glycosylation. [PDF]

open access: yesJ Am Chem Soc
Harale KR   +7 more
europepmc   +1 more source

Iron-Catalyzed Highly Stereospecific Glycosylation with Glycal Epoxides. [PDF]

open access: yesAngew Chem Int Ed Engl
Zhang XW   +5 more
europepmc   +1 more source

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