Results 31 to 40 of about 20,624 (254)
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Priority hierarchies in subject assignment in English: case of conflation
Revista Alicantina de Estudios Ingleses, 2005 The theoretical model of Functional (Discourse) Grammar initially developed by Simón C. Dik (1997a/b) stresses the relevance of implicational hierarchies in grammatical operations and claims that these hierarchies, although they present individual ...
Rodríguez Juárez, Carolina
doaj +1 more source
Pathways and pitfalls: a qualitative study of student experiences in biomedical science education
FEBS Open Bio, EarlyView.Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell +8 more
wiley +1 more source
The influence of grammatical gender on cognition: the case of German and Farsi
Open LinguisticsThis study investigates whether and how grammatical gender influences cognition by comparing speakers of German, a language with three grammatical genders (masculine, feminine, neutral), and Farsi, a genderless language.
Fatemi Maryam +2 more
doaj +1 more source
Atti del Sodalizio Glottologico Milanese, 2014 Active, middle, and passive, as diatheses, are currently (and traditionally) defined in terms of the presumed semantic properties of their subjects. This paper spotlights limits and inadequacies of this viewpoint and claims the functional and syntactic ...
Nunzio La Fauci
doaj +1 more source
Methods for constructing anattribute of an extensively finite predicative, semantically fuzzy set [PDF]
SHS Web of Conferences, 2019 The article looks into the lexical and grammatical characteristics of the system that helps describe an extensively finite predicative and semantically fuzzy set.
Mirgorodskaya Valentina +2 more
doaj +1 more source
MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis
FEBS Open Bio, EarlyView.MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss +7 more
wiley +1 more source
L2 Knowledge of the Obligatory French Subjunctive: Offline Measures and Eye Tracking Compared
Languages, 2021 Extensive research has shown that second language (L2) learners find it difficult to apply grammatical knowledge during real-time processing, especially when differences exist between the first (L1) and L2.
Amber Dudley, Roumyana Slabakova
doaj +1 more source