Results 121 to 130 of about 5,220,012 (288)

Graph Signal Processing: Foundations and Emerging Directions [From the Guest Editors]

open access: yes, 2020
The articles in this special section focus on graph signal processing. Generically, the networks that sustain our societies can be understood as complex systems formed by multiple nodes, where global network behavior arises from local interactions ...
Moura, Jose M.F.   +10 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Signal Processing on Directed Graphs

open access: yes, 2020
This paper provides an overview of the current landscape of signal processing (SP) on directed graphs (digraphs). Directionality is inherent to many real-world (information, transportation, biological) networks and it should play an integral role in processing and learning from network data.
Marques, Antonio G.   +2 more
openaire   +2 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

open access: yesFEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro   +1 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

Graph-Based EEG Signal Compression for Human–Machine Interaction

open access: yesIEEE Access
Communication of bioelectric signals, such as electroencephalography (EEG) signals, will be a key technology for smooth interaction between users and remote robots.
Takuya Fujihashi, Toshiaki Koike-Akino
doaj   +1 more source

Local stationarity of graph signals: insights and experiments

open access: yes, 2017
International audienceIn this paper, we look at one of the most crucial ingredient to graph signal processing: the graph. By taking a step back on the conventional approach using Gaussian weights, we are able to obtain a better spectral representation of
Shrikanth S. Narayanan   +5 more
core   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

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