Results 231 to 240 of about 5,647,687 (283)

Microwave-assisted eco-friendly synthesis of carbon quantum dots derived Cichorium intybus for fluorometric detection of cefdinir. [PDF]

Sci Rep
Khafagy ES, Lila ASA, Ashmawy AM, Al Saqr A, Aldawsari MF, Abdel-Raoof AM, Abdel-Raoof AM.   +6 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

First UV-Based Strategies Integrating Whiteness, Greenness, and Blueness Appraisals for Estimating Meloxicam and Tramadol Concurrently in Their Veterinary Mixture. [PDF]

Arch Pharm (Weinheim)
Mohamed AR, Darweish E, Attia SM, Singh T, Metias YM.   +4 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Sustainable miniaturized smartphone-coupled TLC platform for innovative cleaning validation: application to tizanidine combinations under challenging concentration ratios. [PDF]

Sci Rep
Abd El-Aziz MO, Nadim AH, Monir HH, Nebsen M, Younis SE.   +4 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Green spectrofluorimetric method using nitrogen-phosphorus carbon quantum dots and Box-Behnken optimized salting-out assisted extraction for valbenazine determination in environmental samples. [PDF]

Sci Rep
Serag A, Alosaimi ME, Abduljabbar MH, Baryyan AO, Al Khzem A, Alturki MS, Aldawsari MF, Almalki AH.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Organic solvent-free micellar liquid chromatographic determination of amoxicillin, chlorzoxazone, and ibuprofen in pharmaceutical and biological matrices. [PDF]

Sci Rep
Kabil NA, Zakaria M, Khalil HM, Abdulwahab S.   +3 more
europepmc   +1 more source

AQbD-Based Development and Optimization of a Stability-Indicating RP-HPLC Method for Imeglimin With Integration of Lean Six Sigma Principles. [PDF]

Biomed Chromatogr
Bhusari VK, Ghorpade S, Sethy K.
europepmc   +1 more source