Results 181 to 190 of about 1,535 (215)

Reliable use of green fluorescent protein in fluorescent pseudomonads

Journal of Microbiological Methods, 2001
When fluorescent pseudomonads are cultured on standard solid media under iron limiting conditions, they produce fluorescent, pigmented iron collating agents (siderophores). Siderophores can be readily identified by strong fluorescence seen under UV/blue light.
T M, Timms-Wilson, M J, Bailey
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Green Fluorescent Protein

2005
BACKGROUND. The Discovery of Green Fluorescent Protein. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN. Biochemical and Physical Properties of Green Fluorescent Protein.
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The conformational polymorphism of the green fluorescent protein

Molecular Biology, 2012
Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel ...
Haidong, Tan, Yueguang, Li, Ling, Chen, Takayuki, Kudoh, Tomonari, Kasai, Masaharu, Seno   +5 more
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Green fluorescent protein inspired fluorophores

Advances in Colloid and Interface Science, 2020
Green fluorescence proteins (GFP) are appealing to a variety of biomedical and biotechnology applications, such as protein fusion, subcellular localizations, cell visualization, protein-protein interaction, and genetically encoded sensors. To mimic the fluorescence of GFP, various compounds, such as GFP chromophores analogs, hydrogen bond-rich proteins,
Jia, Kong, Yuefei, Wang, Wei, Qi, Meimei, Huang, Rongxin, Su, Zhimin, He   +5 more
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Structure of cyclized green fluorescent protein

Acta Crystallographica Section D Biological Crystallography, 2002
Crystals of cyclic green fluorescent protein (cGFP) engineered by the previously reported split intein technology [Iwai et al. (2001), J. Biol. Chem. 276, 16548-16554] were obtained and the structure was solved using molecular replacement. Although the core of the protein can unambiguously be fitted from the first to the last residue of the genuine ...
Hofmann, A, Iwai, H, Hess, S, Pluckthun, A, Wlodawer, A   +4 more
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The dark side of green fluorescent protein

New Phytologist, 2005
Summary Here, severe interference of chlorophyll with green fluorescent protein (GFP) fluorescence is described for medicago (Medicago truncatula), rice (Oryza sativa) and arabidopsis (Arabidopsis thaliana). This interference disrupts the proportional relationship between GFP content and fluorescence that is intrinsic to its use as a quantitative ...
Xin, Zhou, Raul, Carranco, Stanislav, Vitha, Timothy C, Hall   +3 more
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Chromophore Formation in Green Fluorescent Protein

Biochemistry, 1997
The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria forms an intrinsic chromophore through cyclization and oxidation of an internal tripeptide motif [Prasher, D. C., et al. (1992) Gene 111, 229-233; Cody, C. E., et al. (1993) Biochemistry 32, 1212-1218].
B G, Reid, G C, Flynn
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Charge transfer in green fluorescent protein

Photochemical & Photobiological Sciences, 2006
Charge transfer reactions that contribute to the photoreactions of the wild type green fluorescent protein (GFP) do not occur in the isolated p-hydroxybenzylidene-imidazolidinone chromophore, demonstrating the role of the protein environment. The high quantum efficiency of the fluorescence photocycle that includes excited state proton transfer and the ...
Jasper J, van Thor, J Timothy, Sage
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The discovery of aequorin and green fluorescent protein

Journal of Microscopy, 2005
We discovered aequorin and green fluorescent protein (GFP) in 1961 from the same species of jellyfish (Shimomura et al ., 1962). Our target was a luminescent substance, aequorin, and GFP was isolated as a by-product of aequorin owing to its bright conspicuous fluorescence.
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Structure and dynamics of green fluorescent protein

Current Opinion in Structural Biology, 1997
Many marine organisms are luminescent. The proteins that produce the light include a primary light producer (aequorin or luciferase) and often a secondary photoprotein that red shifts the light for better penetration in the ocean. Green fluorescent protein is one such secondary protein. It is remarkable in that it autocatalyzes the formation of its own
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