Results 161 to 170 of about 20,936,693 (304)
Exhibition announcement: 2 American Artists, Open Space, Victoria, British Columbia, 1985
, 1985 Printed exhibition announcement for 2 American Artists at Open Space Gallery, British Columbia, Vancouver, Canada, in May and June, 1985.
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Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
Molecular Oncology, EarlyView.How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim +11 more
wiley +1 more source
Management Policy of Green Open Space in Urban Area [PDF]
, 2019 Part of urban space that contains crops, plants and vegetation is known as the Green Open Space (GOS). Several urban areas, with extensive changes and undeveloped land, require the City Green Open Space (CGOS), which can be a 'counterweight' to the ...
Kurniasih, Dewi, Prihandini, Asih
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FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
The costs and benefits of providing open space in cities [PDF]
We use the monocentric model of a city to derive a simple cost-benefit rule for the optimal provision of open space. Although many researchers have investigated the value of open space in cities, few of them have compared it to the costs of providing ...
Jan Rouwendal +1 more
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Production of space in the green open space at Universitas Mercu Buana
IOP Conference Series: Materials Science and Engineering, 2018 S.P. Tunjung Atmadi, Ali Ramadhan
openaire +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source