ICG-guided bowel preservation during emergency TAPP repair for incarcerated Spigelian hernia: a case report. [PDF]
Sakurai H +9 more
europepmc +1 more source
Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Working with objective and perceived indicators of health and green space
Objective or “actual” access to green spaces near homes is not the same as perceived access to green space. Similarly, objective health, such as absence of disease, is not necessarily equivalent to how people perceive their health.
Nordh, Helena, +1 more
core
Urban environmental exposure as a factor in adaptive neuromodulation: a conceptual framework. [PDF]
Qamili A, Cenameri A.
europepmc +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
From past, persisting, and consolidating ecologies of segregation to ecologies of repair. [PDF]
Anguelovski I +7 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Objectively Measured Life Space Using Participants' Mobile Phones: Cross-Sectional Study. [PDF]
Odden MC +10 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Foundation model enables interpretable open and error-tolerant searching for mass spectrometry-based proteomics. [PDF]
Altenburg T +4 more
europepmc +1 more source

