Results 311 to 320 of about 60,004 (331)

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function

Nature Chemical Biology, 2018
Predictable control over gene expression is essential to elicit desired synthetic cellular phenotypes. Although CRISPR-Cas9 offers a simple RNA-guided method for targeted transcriptional control, it lacks the ability to integrate endogenous cellular information for efficient signal processing.
Ka-Hei Siu, Wilfred Chen
exaly   +4 more sources

The yeast platform engineered for synthetic gRNA-landing pads enables multiple gene integrations by a single gRNA/Cas9 system.

Metabolic Engineering, 2021
Saccharomyces cerevisiae is a versatile microbial platform to build synthetic metabolic pathways for production of diverse chemicals. To expedite the construction of complex metabolic pathways by multiplex CRISPR-Cas9 genome-edit, eight desirable ...
Sihyun Baek, J. C. Utomo, J. Lee, Kunali Dalal, Y. Yoon, D. Ro   +5 more
semanticscholar   +3 more sources

Friend or Foe? Evidence Indicates Endogenous Exosomes Can Deliver Functional gRNA and Cas9 Protein

Small, 2019
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated nuclease (Cas) system is an efficient gene editing tool. In this study, it is found that both single guide RNA (gRNA) and Cas9 protein could be exported from the CRISPR ...
Ting Zhang, Xiangmei Chen, Fengmin Lu
exaly   +2 more sources

Synthetic gRNA/Cas9 ribonucleoprotein targeting HBV DNA inhibits viral replication

Journal of Medical Virology, 2023
The presence of hepatitis B virus (HBV) covalently closed circular (ccc) DNA (cccDNA), which serves as a template for viral replication and integration of HBV DNA into the host cell genome, sustains liver pathogenesis and constitutes an intractable ...
Jinyu Zhang, Y. Zhang, Sushant Khanal, Dechao Cao, Juan Zhao, Xindi Dang, Lam N. Nguyen, M. Schank, Xiao Y. Wu, Yongfa Jiang, Shunbin Ning, Ling Wang, M. El Gazzar, J. Moorman, Haitao Guo, Zhiqiang Yao   +15 more
semanticscholar   +1 more source

gRNA-transient expression system for simplified gRNA delivery in CRISPR/Cas9 genome editing

Journal of Bioscience and Bioengineering, 2019
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) system is one of the most powerful tools for genome engineering. However, some of the steps are laborious, reducing its usability. In this study, we have developed a simplified method, called the guide RNA-transient expression system ...
Farhana Easmin, Naim Hassan, Yu Sasano, Keisuke Ekino, Hisataka Taguchi, Satoshi Harashima   +5 more
openaire   +2 more sources

CRISPR-gRNA Design

2019
Gene editing has great therapeutic impact, being of interest for many scientists worldwide. Clustered regularly interspaced short palindromic repeats (CRISPR) technology has been adapted for gene editing to serve as an efficient, rapid, and cost-effective tool.
Maria, Pallarès Masmitjà, Nastassia, Knödlseder, Marc, Güell   +2 more
openaire   +2 more sources

Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system

Nature Protocols, 2022
Siqi Li, Hao Wu, Ling-Ling Chen
semanticscholar   +1 more source

Genomic allele-specific base editing with imperfect gRNA

Journal of Genetics and Genomics, 2023
Xuxu Chen, Dongdong Zhao, Xueting Hou, Ju Li, Shiming Pu, Jidong Fei, Siwei Li, Zuping Zhou, Changhao Bi, Xueli Zhang   +9 more
openaire   +2 more sources

Genome aware CRISPR gRNA target prediction for parasitic nematodes

Molecular and Biochemical Parasitology, 2019
The pace of research towards a genetic model to understand the unique molecular biology of parasitic nematodes has increased recently. This research has developed a diverse suite of genetic tools for a variety of parasitic nematodes. CRISPR/Cas9 technology in particular offers much promise as a game changing tool for researchers studying parasitic ...
openaire   +2 more sources