Results 321 to 330 of about 60,004 (331)
Some of the next articles are maybe not open access.
Local sliding PAMs alter CRISPR/Cas9 gRNA activity
2021A major challenge of CRISPR/Cas9-mediated genome engineering is that not all guide RNAs cleave DNA efficiently. Here, we show that local gRNA-DNA interactions resulting from Cas9 sliding on overlapping protospacer adjacent motifs profoundly impact gRNA activities.
openaire +1 more source
Programmable RNA base editing with a single gRNA-free enzyme
Nucleic Acids Research, 2022Zefeng Wang
exaly
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease
Nucleic Acids Research, 2013Wataru Fujii, Koji Sugiura
exaly
Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries
Nature Communications, 2015Joana Alves Vidigal, Andrea Ventura
exaly
Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX
Biotechnology Letters, 2016Xiquan Liang, Shantanu Kumar
exaly