In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Online TCP Throughput Map Maintenance Under Budget-Constrained Vehicular Sensing. [PDF]
Sensors (Basel)Hu W, Ohsita Y, Shimonishi H.
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Development of a Semi-Automated Tool Based on Computer Vision Methods for Safety Assessment of Micromobility Users. [PDF]
Sensors (Basel)Fonseca-Cabrera AS +2 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
A Japanese-Dietitian Prompt Systematically Shifts Portion Estimates in LLM-Based Nutrient Estimation from Food Images: A Multi-Dataset, Multi-Model Study. [PDF]
NutrientsNakagawa S, Yamamoto A.
europepmc +1 more source
Ground water pollution potential of Cuyahoga County, Ohio
, 1994 "April, 1994."; Includes errata sheet; Includes bibliographical references (pages 24 ...
Barber, Douglas J.
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FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Assessing the Accuracy and Completeness of OpenStreetMap Sidewalks: A Comparative Study With Google Street View. [PDF]
Prev Chronic DisNichols BL, Groth C, Quinn TD.
europepmc +1 more source
Five Points Lutheran, 1861-1961.
, 1961 Centennial Festivities program tipped-in at back.Cover title.Mode of access ...
Five Points Lutheran Church (Blue River, Wis.)
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