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Controlling Supramolecular Fiber Formation of Nucleopeptide by Guanosine Triphosphate
Biomacromolecules, 2023Cells use dynamic self-assembly to construct functional structures for maintaining cellular homeostasis. However, using a natural biological small molecule to mimic this phenomenon remains challenging. This work reports the dynamic microfiber formation of nucleopeptide driven by guanosine triphosphate, the small molecule that controls microtubule ...
Hongyue Zhang +7 more
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[37] Guanosine triphosphate-8-formylhydrolase
1975Publisher Summary This chapter discusses the assay and purification procedure of guanosine triphosphate-8-formylhydrolase. The assay method is based on two procedures. The first method involves the release of 14 C-labeled formic acid from carbon-8 of the imidazole ring of [8- 14 C]GTP. The formic acid is oxidized to carbon dioxide. The second method
R.J. Suhadolnik, E.F. Elstner
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Direct photoaffinity labeling of tubulin with guanosine 5'-triphosphate
Biochemistry, 1985Irradiation of tubulin in the presence of [3H]GTP or [3H]GDP at 254 nm led to the covalent incorporation of nucleotide into the protein. The specific nature of the labeling was shown in the following manner: with tubulin depleted of exchangeable nucleotide, the amount of labeling increased to a plateau value as the [3H]GTP concentration was increased ...
Richard H. Himes +2 more
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Preparative method of obtaining guanosine 5′-triphosphate
Chemistry of Natural Compounds, 19651. The kinetics of the formation of guanosine 5′-triphosphate, 5′-diphosphate, and 5′-monophosphate in the reaction of the morpholide of guanosine 5′-monophosphate with bis-(tributylammonium) pyrophosphate in pyridine at 27° has been recorded. 2. In the reaction of the morpholide of guanosine 5′-monophosphate with bis-(tributylammonium) pyrophosphate ...
T. S. Lomakina, N. I. Grineva
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Evidence for Guanosine Triphosphate—Binding Proteins in Trypanosoma cruzi
The American Journal of Tropical Medicine and Hygiene, 1994The transformation of the parasite Trypanosoma cruzi from the blood-borne trypomastigote to the intracellular amastigote constitutes a key clinical feature in the pathophysiology of Chagas' disease. That this transition occurs without change in the integrity of the plasma membrane of the parasite suggests the presence of biochemical structures, i.e ...
Huan Huang +5 more
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Adenosine Triphosphate and Guanosine Triphosphate Determinations in Intertidal Sediments
1979Measurements were made of the spatial distributions of adenosine triphosphate (ATP) on a variety of sandy beaches. Samples were collected during ebb flow and extracted in the field using cold sulfuric acid and ethylenediamine tetraacetate (EDTA); the neutralized extracts were immediately frozen for subsequent analyses.
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On the mechanism of guanosine triphosphate hydrolysis in ras p21 proteins
Biochemistry, 1992The residue Gln61 is assumed to play a major role in the mechanism of ras p21, and mutations of this residue are often found in human tumors. Such mutations lead to a major reduction in the rate of GTP hydrolysis by the complex of ras p21 and the GTPase activating protein (GAP) and lock the protein in a growth-promoting state.
Ralf Langen +2 more
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The effect of guanosine 5′-triphosphate analogues on protein synthesis
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971Abstract Four GTP analogues, 6-mercaptoguanosine 5′-triphosphate (I), N2-methylguanosine 5′-triphosphate (II), N2-dimethylguanosine 5′-triphosphate (III) and 8-bromoguanosine 5′-triphosphate, (IV) were synthesized chemically and tested for their ability to replace GTP in the cell-free amino acid incorporation system of Escherichia coli.
Morio Ikehara +3 more
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Biochemistry, 1986
Using highly purified calf brain tubulin bearing [8-14C]guanosine 5'-diphosphate (GDP) in the exchangeable nucleotide site and heat-treated microtubule-associated proteins (both components containing negligible amounts of nucleoside diphosphate kinase and nonspecific phosphatase activities), we have found that a significant proportion of exchangeable ...
Janendra K. Batra +2 more
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Using highly purified calf brain tubulin bearing [8-14C]guanosine 5'-diphosphate (GDP) in the exchangeable nucleotide site and heat-treated microtubule-associated proteins (both components containing negligible amounts of nucleoside diphosphate kinase and nonspecific phosphatase activities), we have found that a significant proportion of exchangeable ...
Janendra K. Batra +2 more
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Structural and Functional Relationships of Guanosine Triphosphate Binding Proteins
1988Information available at present documents the existence of three well-defined classes of guanine nucleotide binding proteins functioning as signal transducers: Gs and Gi which stimulate and inhibit adenylate cyclase, respectively, and transducin which transmits and amplifies the signal from light-activated rhodopsin to cGMP-dependent phosphodiesterase
Ernst J.M. Helmreich, Thomas Pfeuffer
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