Results 311 to 320 of about 4,450,920 (398)

Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276

FEBS Open Bio, EarlyView.
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig, Swanhild Lohse, Sara Elahi, Nils Hartmann, Stefanie Deckert, Shutian Si, Alexander Desuki, Anja Harder   +7 more
wiley   +1 more source

Combining oligo pools and Golden Gate cloning to create protein variant libraries or guide RNA libraries for CRISPR applications [PDF]

Alicia Maciá Valero, Rianne C. Prins, Thijs de Vroet, Sonja Billerbeck   +3 more
openalex   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex. [PDF]

Mol Cell
Wang PY, Bartel DP.
europepmc   +1 more source

Preventing large deletions and chromosome loss in engineered human primary T cells by CasPlus with optimized guide RNAs [PDF]

Qiaoyan Yang, Jonathan S. Abebe, Michelle Mai, Colin T. Konishi, Orrin Devinsky, Chengzu Long   +5 more
openalex   +1 more source

TMC4 localizes to multiple taste cell types in the mouse taste papillae

FEBS Open Bio, EarlyView.
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata, Yoshikazu Saito, Yoichi Kasahara, Wataru Fujii, Takumi Misaka, Tomiko Asakura, Masataka Narukawa   +6 more
wiley   +1 more source

CRISPR-Cas guide RNA indel analysis using CRISPResso2 with Nanopore sequencing data. [PDF]

BMC Res Notes
McFarlane GR, Polanco JVC, Bogema D.
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Re-engineered guide RNA enables DNA loops and contacts modulating repression in E. coli. [PDF]

Nucleic Acids Res
Yang Y, Rocamonde-Lago I, Shen B, Berzina I, Zipf J, Högberg B.   +5 more
europepmc   +1 more source

Author response: Integrated Analysis of Single-Cell and Bulk RNA-Seq Data reveals that Ferroptosis-Related Genes Mediated the Tumor Microenvironment predicts Prognosis, and guides Drug Selection in Triple-Negative Breast Cancer

Xuantong Gong, Lishuang Gu, Di Yang, Yu He, Qian Li, Hao Qin, Yong Wang   +6 more
openalex   +1 more source