Results 331 to 340 of about 570,529 (349)
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Chimeric DNA–RNA Guide RNA Designs
2020CRISPR-associated nuclease (Cas) has been widely applied to modify the genomes of various cell types. As RNA-guided endonucleases, Cas enzymes can target different genomic sequences simply by changing the guide sequence of the CRISPR RNA (crRNA) or single guide RNA (sgRNA).
Shuhan Lu, Hao Yin, Ying Zhang
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Trends in Genetics, 1999
Cold Spring Harbor Laboratory Press, 1999. $129.00 (709 + xxv pages)ISBN 0 87969 561 7This is a new edition of a multi-author work whose first edition appeared in 1993. To quote from Crick’s foreword to the first edition (reprinted in this edition), ‘the “RNA World” originally referred to a hypothetical time in the evolution of earthly life when there ...
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Cold Spring Harbor Laboratory Press, 1999. $129.00 (709 + xxv pages)ISBN 0 87969 561 7This is a new edition of a multi-author work whose first edition appeared in 1993. To quote from Crick’s foreword to the first edition (reprinted in this edition), ‘the “RNA World” originally referred to a hypothetical time in the evolution of earthly life when there ...
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External Guide Sequences for an RNA Enzyme [PDF]
Science, 1990 Ribonuclease P (RNase P) from Escherichia coli or its catalytic RNA subunit can efficiently cleave small RNA substrates that lack the conserved features of natural substrates of RNase P if an additional small RNA is also present.
Anthony C. Forster, Sidney Altman
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Characterization of Cas9–Guide RNA Orthologs [PDF]
Cold Spring Harbor Protocols, 2016 In light of the multitude of new Cas9-mediated functionalities, the ability to carry out multiple Cas9-enabled processes simultaneously and in a single cell is becoming increasingly valuable. Accomplishing this aim requires a set of Cas9–guide RNA (gRNA) pairings that are functionally independent and insulated from one another.
Stephanie J. Yaung +6 more
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The principles of guiding by RNA: chimeric RNA–protein enzymes
Nature Reviews Genetics, 2006The non-protein-coding transcriptional output of the cell is far greater than previously thought. Although the functions, if any, of the vast majority of these RNA transcripts remain elusive, out of those for which functions have already been established, most act as RNA guides for protein enzymes.
Peter Schattner, Alexander Hüttenhofer
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RNA-Guided RNA Modifications: Biogenesis, Functions, and Applications
Accounts of Chemical Research, 2023ConspectusPost-transcriptional modifications are ubiquitous in both protein-coding and noncoding RNAs (ncRNAs), playing crucial functional roles in diverse biological processes across all kingdoms of life. These RNA modifications can be achieved through two distinct mechanisms: RNA-independent and RNA-guided (also known as RNA-dependent).
Bin Li, Lianghu Qu, Jianhua Yang
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Nature Biotechnology, 2013
The Cas9 endonuclease is reprogrammed by RNAs for site-specific modification of eukaryotic and microbial genomes.
Mussolino, Claudio, Cathomen, Toni
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The Cas9 endonuclease is reprogrammed by RNAs for site-specific modification of eukaryotic and microbial genomes.
Mussolino, Claudio, Cathomen, Toni
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A guide to small RNAs in microorganisms
Current Opinion in Microbiology, 2007www.sciencedirect.com Overview When regulation of the lactose metabolism genes was first discovered in Escherichia coli, Jacob and Monod [1] proposed that the regulator was an RNA that would block the expression of lac mRNA at the lac operator. This proposal was largely forgotten with the discovery of the LacI repressor protein, along with the hundreds
Dieter Haas, Gisela Storz
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RNA Editing: RNA duplexes guide base conversions
Current Biology, 1994Recent evidence strengthens the case that editing of mRNAs encoding glutamate receptors subunits occurs by a mechanism in which the RNA editing substrate has a double-stranded conformation.
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Small Nucleolar RNAs Guide Ribosomal RNA Methylation
Science, 1996Two recent reports, one in Cell and one in the Journal of Molecular Biology , show that the newly described small nucleolar RNAs control the site of methylation of the ribosomal RNA (rRNA). Tollervey explains how these small RNAs accomplish this targeting and discusses the possible role of ...
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