Results 171 to 180 of about 5,137 (188)

Fructose transport byHaloferax volcanii

Canadian Journal of Microbiology, 1995
Uptake of fructose by intact cells of Haloferax volcanii, one of the sugar-utilizing halobacteria, was examined with the following results. (i) The fructose transporter was inducible, (ii) Kinetic analysis showed a Ktof 0.37 μM and a Vmaxof 4.61 nmol∙mg protein−1∙min−1.
Jin-Ichiro Takano, Kouichi Kaidoh, Naoki Kamo   +2 more
openaire   +1 more source

Nutrition of the Halophilic Archaebacterium, Haloferax volcanii

Systematic and Applied Microbiology, 1990
Summary A synthetic medium containing glycerol and succinate as carbon sources, NH4Cl as nitrogen source, thiamine and biotin, as well as salts, supports growth of Haloferax volcanii and other halophilic archaebacteria. A number of single carbon compounds can also support growth, as can single nitrogen compounds (urea, histidine and glutamate).
Tiiu Kauri, Rebecca Wallace, Donn J. Kushner   +2 more
openaire   +1 more source

In Vivo Protein Cross-Linking and Coimmunoprecipitation in Haloferax volcanii

2022
Coimmunoprecipitation is a powerful and commonly used method to identify protein-protein interactions in a physiological context. Here, we report a coimmunoprecipitation protocol that was adapted and optimized for the haloarchaeon Haloferax volcanii to identify interacting partners to the LonB protease.
Paggi, Roberto Alejandro, de Castro, Rosana Esther, Cerletti, Micaela   +2 more
openaire   +3 more sources

Novel glycoproteins of the halophilic archaeon Haloferax volcanii

Archives of Microbiology, 2000
Archaea possess many eukaryote-like properties, including the ability to glycosylate proteins. Using oligosaccharide staining and lectin binding, this study revealed the existence of several glycosylated Haloferax volcanii membrane proteins, besides the previously reported surface layer (S-layer) glycoprotein.
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The 2-oxoacid dehydrogenase multienzyme complex of Haloferax volcanii

Extremophiles, 2007
Those aerobic archaea whose genomes have been sequenced possess four adjacent genes that, by sequence comparisons with bacteria and eukarya, appear to encode the component enzymes of a 2-oxoacid dehydrogenase multienzyme complex. However, no catalytic activity of any such complex has ever been detected in the archaea.
Dina M, Al-Mailem, David W, Hough, Michael J, Danson   +2 more
openaire   +2 more sources

Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii

2022
Biofilms are cellular aggregates encased in extracellular polymeric substances and are commonly formed by single-celled eukaryotes, bacteria, and archaea. In addition to attaching to solid surfaces, these cellular aggregates can also be observed floating on or immersed within liquid cultures. While biofilms on surfaces have been studied in some archaea,
Zuha, Mutan, Heather, Schiller, Stefan, Schulze, Mechthild, Pohlschroder   +3 more
openaire   +2 more sources

Small RNAs of the halophilic archaeon Haloferax volcanii

Biochemical Society Transactions, 2009
In recent years, sRNAs (small non-coding RNAs) have been found to be abundant in eukaryotes and bacteria and have been recognized as a novel class of gene expression regulators. In contrast, much less is known about sRNAs in archaea, except for snoRNAs (small nucleolar RNAs) that are involved in the modification of bases in stable RNAs.
Jörg, Soppa, Julia, Straub, Mariam, Brenneis, Angelika, Jellen-Ritter, Ruth, Heyer, Susan, Fischer, Michaela, Granzow, Björn, Voss, Wolfgang R, Hess, Brian, Tjaden, Anita, Marchfelder   +10 more
openaire   +2 more sources

A Protein-Serine Phosphatase from the Halophilic Archaeon Haloferax volcanii

Biochemical and Biophysical Research Communications, 1993
We have detected a protein phosphatase activity in soluble extracts from the halophilic archaeon Haloferax volcanii. This activity was markedly stimulated by the divalent metal ions Mn2+ and Cd2+. It dephosphorylated phosphoseryl residues in casein, mixed histones, and phosphorylase a, but not phosphotyrosyl residues in reduced, carboxyamidomethylated ...
K A, Oxenrider, P J, Kennelly
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Amber suppression in the archaebacterium Haloferax volcanii

2009
The purpose of this project was to test whether amber suppression can occur in Haloferax volcanii or not, and if so, to construct a H. volcanii strain that can suppress amber mutations. To achieve this goal, a putative amber suppressor was constructed from the tyrosine transfer RNA of H. volcanii.
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Haloferax volcanii DS2 and Halobacterium salinarium GRB

1998
Contig maps for two haloarchaeal genomes have been constructed to date: Haloferax volcanii DS2 (Charlebois et al., 1991) and Halobacterium salinarium GRB (St. Jean et al., 1994). Additionally, chromosomal macrorestriction maps are available from Haloferax mediterranei ATCC 33500 (Lopez-Garcia et al., 1992; Anton et al., 1994) and from Hb.
openaire   +1 more source