Optogenetic approaches for neural tissue regeneration: A review of basic optogenetic principles and target cells for therapy [PDF]
Neural Regeneration ResearchOptogenetics has revolutionized the field of neuroscience by enabling precise control of neural activity through light-sensitive proteins known as opsins.
Davletshin Eldar +5 more
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Light-Driven Chloride Transport Kinetics of Halorhodopsin [PDF]
Biophysical Journal, 2018 Despite growing interest in light-driven ion pumps for use in optogenetics, current estimates of their transport rates span two orders of magnitude due to challenges in measuring slow transport processes and determining protein concentration and/or orientation in membranes in vitro.
Hasin Feroz +17 more
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Charge Motions During the Photocycle of pharaonis Halorhodopsin [PDF]
Biophysical Journal, 2000 Oriented gel samples were prepared from halorhodopsin-containing membranes from Natronobacterium pharaonis, and their photoelectric responses to laser flash excitation were measured at different chloride concentrations. The fast component of the current signal displayed a characteristic dependency on chloride concentration, and could be interpreted as ...
Krisztina Ludmann +3 more
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Reconstitution of purified halorhodopsin. [PDF]
Proceedings of the National Academy of Sciences, 1984 Asolectin lipid vesicles containing halorhodopsin show light-induced acidification in the presence of proton ionophores. This effect is abolished by triphenyltin chloride, a chloride/hydroxyl antiporter, and is greatly diminished by valinomycin in the presence of potassium ions, which collapse the membrane potential.
Roberto A. Bogomolni +2 more
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eNpHR: a Natronomonas halorhodopsin enhanced for optogenetic applications [PDF]
Brain Cell Biology, 2008 Temporally precise inhibition of distinct cell types in the intact nervous system has been enabled by the microbial halorhodopsin NpHR, a fast light-activated electrogenic Cl(-) pump. While neurons can be optically hyperpolarized and inhibited from firing action potentials at moderate NpHR expression levels, we have encountered challenges with pushing ...
Viviana Gradinaru +2 more
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Nanosecond Transient IR Spectroscopy of Halorhodopsin in Living Cells. [PDF]
J Am Chem SocOldemeyer S +5 more
europepmc +3 more sources
Important amino acid residues in the chloride pump halorhodopsin that accelerate ion transport despite no direct interaction with the substrate. [PDF]
J Biol ChemZhai Y +3 more
europepmc +3 more sources
Halorhodopsin - Light-driven Cl--pump.
membrane, 1984 Tôru Yoshizawa, Tarou Ogurusu
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Roles of basic amino acid residues in substrate binding and transport of the light-driven anion pump Synechocystis halorhodopsin (SyHR). [PDF]
J Biol ChemNakama M +5 more
europepmc +2 more sources
Light Stimulation Parameters Determine Neuron Dynamic Characteristics
Applied Sciences, 2019 Optogenetics is a recently developed technique that is widely used to study neuronal function. In optogenetic experiments, neurons encode opsins (channelrhodopsins, halorhodopsins or their derivatives) by means of viruses, plasmids or genetic ...
Alexander Erofeev +6 more
doaj +1 more source