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Proton Transport by Halorhodopsin

Biochemistry, 1996
In halorhodopsin from Natronobacterium pharaonis, a light-driven chloride pump, the chloride binding site also binds azide. When azide is bound at this location the retinal Schiff base transiently deprotonates after photoexcitation with light > 530 nm, like in the light-driven proton pump bacteriorhodopsin.
G, Váró   +3 more
openaire   +2 more sources

Secondary structure of halorhodopsin

Biochemistry, 1986
Ultraviolet circular dichroism (CD) spectroscopy in the interval from 190 to 240 nm has been used to estimate the secondary structural content of halorhodopsin (hR), a light-driven chloride pump isolated from the membranes of Halobacterium halobium. Least-squares curve fitting of the CD spectrum for hR solubilized with octyl glucoside yields an alpha ...
B K, Jap, S H, Kong
openaire   +2 more sources

Cation Binding to Halorhodopsin

Biochemistry, 2015
A member of the retinal protein family, halorhodopsin, acts as an inward light-driven Cl(-) pump. It was recently demonstrated that the Natronomonas pharaonis halorhodopsin-overproducing mutant strain KM-1 contains, in addition to the retinal chromophore, a lipid soluble chromophore, bacterioruberin, which binds to crevices between adjacent protein ...
Sansa, Dutta   +2 more
openaire   +2 more sources

Light and dark adaptation of halorhodopsin

Archives of Biochemistry and Biophysics, 1985
Dark incubation of envelope vesicles derived from a strain of Halobacterium halobium that lacks bacteriorhodopsin but contains halorhodopsin and a third rhodopsin-like pigment caused a decrease in the flash yield [the amplitude of a transient absorbance change of flash reactive component(s) by flash] of halorhodopsin but not the rhodopsin-like pigment.
N, Kamo   +3 more
openaire   +2 more sources

Photochemistry of Halorhodopsin

2015
Halorhodopsin is a light-driven inward Cl− pump found in the membrane of a halophilic archaeon called Halobacterium salinarum. While the physiological role of halorhodopsin has not been fully resolved, its functional mechanism has been studied as a model system for anion transport.
Takashi Kikukawa   +2 more
openaire   +1 more source

Crystallization of Halorhodopsin from Halobacterium sp. shark

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2005
The chloride-ion-pumping channel, halorhodopsin from Halobacterium sp. shark was detergent-solubilized and 3-D crystallized. Proteins were solubilized using the nonionic detergent n-octyl-beta-D-glucoside and were crystallized as thin-plate crystals with polyethylene glycol 4000 as a precipitant.
Hirokazu, Nishida   +3 more
openaire   +2 more sources

Bacteriorhodopsin and halorhodopsin: Multiple ion pumps

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
Des membranes pourpres d'Halobacterium halobium contenant la bacteriorhodopsine ont ete soumis a des pulses laser ou a une irradiation continue en lumiere visible. En fonction du pH, la capacite et la specificite du transport d'ions par la bacteriorhodopsine et l'halorhodopsine sont ...
L. Keszthelyi   +3 more
openaire   +1 more source

Halorhodopsin: A Light-Driven Chloride Ion Pump

Annual Review of Biophysics and Biomolecular Structure, 1986
Studies of bacteriorhodopsin over the past fourteen years have contributed in a special special way to our present understanding of ionic pumps and membrane proteins in general. This is because bacteriorhodopsin, a light­ driven proton pump found in the halo bacteria, is a small membrane protein that contains the minimum features required for active ...
openaire   +2 more sources

Robust Nonlinear Control for Synchronising and Regulating Neural Activity

Journal of Electronics and Electrical Engineering
Modulating neural activity in a systematic manner holds significant potential for advancing the understanding of brain functions and improving therapeutic strategies.
Sebastián Martínez   +2 more
semanticscholar   +1 more source

Optogenetics and Beyond: Expanding the Frontiers of Brain Science through Light-Based Neural Control

International Journal of Biology and Life Sciences
Optogenetics—light-driven, genetically targeted control of neuronal activity—has transformed neuroscience by allowing precise manipulation of specified neural populations with millisecond timing.
Chenghochi
semanticscholar   +1 more source

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