Results 31 to 40 of about 1,936 (237)

Purification of photochemically active halorhodopsin. [PDF]

Proceedings of the National Academy of Sciences, 1983
We have developed a procedure for the purification of halorhodopsin in a photochemically active state. Solubilization of membranes from a bacteriorhodopsin-negative Halobacterium strain with octyl glucoside was followed by chromatography on hydroxylapatite and octyl-Sepharose gels. All steps were carried out in high-ionic-strength solutions.
M E, Taylor, R A, Bogomolni, H J, Weber
openaire   +2 more sources

Structural Mechanism for Light-driven Transport by a New Type of Chloride Ion Pump, Nonlabens marinus Rhodopsin-3*♦

Journal of Biological Chemistry, 2016
The light-driven inward chloride ion-pumping rhodopsin Nonlabens marinus rhodopsin-3 (NM-R3), from a marine flavobacterium, belongs to a phylogenetic lineage distinct from the halorhodopsins known as archaeal inward chloride ion-pumping rhodopsins. NM-R3
T. Hosaka, Susumu Yoshizawa, Y. Nakajima, N. Ohsawa, M. Hato, E. Delong, K. Kogure, S. Yokoyama, T. Kimura-Someya, W. Iwasaki, M. Shirouzu   +10 more
semanticscholar   +1 more source

Primary charge separation in halorhodopsin [PDF]

FEBS Letters, 1985
INTRODUCTION Halorhodopsin (hR) is a retinal-binding protein in the cell membrane of Halobacterium halobium [l-4]. On the basis of light-induced volume change measurements on cell envelope vesicles, hR has been suggested to act as a light-driven Cl- pump [5].
Dér, A., Fendler, K., Keszthelyi, L., Bamberg, E.   +3 more
openaire   +2 more sources

Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review. [PDF]

Europace
State-of-the-art innovations in optical cardiac electrophysiology are significantly enhancing cardiac research. A potential leap into patient care is now on the horizon.
Baines O, Sha R, Kalla M, Holmes AP, Efimov IR, Pavlovic D, O'Shea C.   +6 more
europepmc   +3 more sources

Functional reconstitution of halorhodopsin. Properties of halorhodopsin-containing proteoliposomes.

Journal of Biological Chemistry, 1988
A one-step purification method for halorhodopsin was developed. Functional proteoliposomes were prepared from this preparation using cholate, which is removed by dialysis in the presence of asolectin or the polar halobacterial lipids. Light-induced outward directed transport of chloride by halorhodopsin was followed by measuring passive proton efflux ...
A, Duschl, M A, McCloskey, J K, Lanyi
openaire   +2 more sources

FEF inactivation with improved optogenetic methods [PDF]

, 2016
Optogenetic methods have been highly effective for suppressing neural activity and modulating behavior in rodents, but effects have been much smaller in primates, which have much larger brains. Here, we present a suite of technologies to use optogenetics
Acker, Leah Christine, Boyden, Edward, Desimone, Robert, Pino, Erica N.   +3 more
core   +2 more sources

Evaluating the outcomes of pluripotent stem-cell-derived photoreceptor transplantation in retinal repair. [PDF]

FEBS J
Transplantation of stem cell‐derived photoreceptors in retinal repair. We summarize methods for differentiating stem cells and describe surgical techniques for transplanting photoreceptor precursors to restore visual function (A). The effectiveness of these techniques is assessed with behavioral (B) and in vitro assays (C) using mouse models that mimic
Zerti D, Dorgau B, Sernagor E, Armstrong L, Lako M, Hilgen G.   +5 more
europepmc   +2 more sources

Optical control of zebrafish behavior with halorhodopsin [PDF]

Proceedings of the National Academy of Sciences, 2009
Expression of halorhodopsin (NpHR), a light-driven microbial chloride pump, enables optical control of membrane potential and reversible silencing of targeted neurons. We generated transgenic zebrafish expressing enhanced NpHR under control of the Gal4/UAS system.
Arrenberg, A., Del Bene, F., Baier, H.
openaire   +3 more sources

Noninvasive optical inhibition with a red-shifted microbial rhodopsin [PDF]

, 2014
Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws,
A Berényi, A Hofherr, A Nimmerjahn, A Roggan, AB Arrenberg, Amy S Chuong, AN Bashkatov, AN Yaroslavsky, Andreas T Sørensen, Andrew Young, BN Armbruster, Botond Roska, Brian D Allen, Brian Y Chow, BY Chow, CA Giller, CH Sung, Craig R Forest, D Huber, D Ma, DB Farber, Edward S Boyden, EJ Yoder, ES Boyden, F Naarendorp, GH Jacobs, Gillian A C Matthews, H Ye, HT Xu, I Ibañez-Tallon, J Grutzendler, J Hatazawa, J Mattis, J Menzler, J Otomo, J Tønnesen, JA Cardin, JA Cardin, JC Grieger, Jessica A Cardin, JT Trachtenberg, JV Raimondo, JY Lin, Kay M Tye, KM Tye, L Madisen, L Wang, Leah C Acker, M Jazayeri, M Rüdiger, Masaaki Ogawa, Mike A Henninger, Mitra L Miri, N Ugryumova, NA Scott, Nathan C Klapoetke, NC Klapoetke, NR Hackett, O Yizhar, P Anikeeva, P Kreiss, P Znamenskiy, PJ Drew, R Hira, Rachel C Bandler, RH Kramer, S Hippenmeyer, S Ohayon, S-H Lee, SB Kodandaramaiah, Shreshtha B Ramanlal, SI Al-Juboori, Suhasa B Kodandaramaiah, T Binzoni, T Tsubota, T Tsunematsu, V Busskamp, V Busskamp, V Gradinaru, V Gradinaru, Volker Busskamp, VS Polikov, X Han, X Han, X Li, Xue Han, Yingxi Lin   +86 more
core   +1 more source

The Boston University Photonics Center annual report 2014-2015 [PDF]

, 2015
This repository item contains an annual report that summarizes activities of the Boston University Photonics Center in the 2014-2015 academic year. The report provides quantitative and descriptive information regarding photonics programs in education ...
The Boston University Photonics Center
core   +1 more source