Results 81 to 90 of about 3,898,099 (355)

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Application of dealloying method in magnetic abrasive finishing of YG20 hard alloy

open access: yesJin'gangshi yu moliao moju gongcheng
ObjectivesThe primary objective of this study is to enhance the machining efficiency of hard alloy by combining electrochemical decarburization with magnetic abrasive finishing (MAF). The goal is to address the challenges in machining hard alloy, such as
Zijian WANG   +4 more
doaj   +1 more source

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

open access: yesFEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

Surface roughness modeling of CBN hard steel turning [PDF]

open access: yes, 2018
Study in the paper investigate the influence of the cutting conditions parameters on surface roughness parameters during turning of hard steel with cubic boron nitrite cutting tool insert. For the modeling of surface roughness parameters was used central
Ješić, D.   +5 more
core  

Application of CrAlN coatings on carbide substrates in routing of MDF [PDF]

open access: yes, 2007
This study deals with the development of Chromium Aluminium Nitride (CrAlN) hard coatings (by varying the nitrogen content in the plasma, the target bias voltage, the working pressure and the deposition time) and their characterization by physical and ...
COLLET, Robert   +4 more
core   +5 more sources

Thermal Loads and Surface Quality Evaluation in Machining of Hardened Die Steel under Dry and Cryogenic Machining [PDF]

open access: yesInternational Journal of Advanced Design and Manufacturing Technology, 2020
AISI H13 die steel is widely used in different industries because of its especial properties. During the machining of hard materials, some of the mechanical properties of the material are changed due to the generation of intensive thermo-mechanical loads
Farshid Jafarian, Emad Mohseni
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Rain water harvesting management system in Zoo Negara, Selangor [PDF]

open access: yes, 2018
Water is a key requirement either to human, animal or plant. Rainwater Harvesting System (RWH) is a technology used to collect and store rainwater from the roof for reuse.
Md Yassin, Azlina   +3 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Electro-Discharge Machining of Ceramics: A Review

open access: yesMicromachines, 2018
Conventional machining techniques of ceramics such as milling, drilling, and turning experience high cutting forces as well as extensive tool wear. Nevertheless, non-contact processes such as laser machining and electro-discharge machining (EDM) remain ...
Azat Bilal   +3 more
doaj   +1 more source

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