An in situ liquid environment for synchrotron hard X-ray nanoprobe microscopy
Studying chemical reactions in an environment that closely mimics the system’s natural operating conditions can offer crucial insights into dynamic oxidation processes. Transmission Electron Microscopes (TEMs)and X-ray Nanoprobes allow the use of imaging and spectroscopy to access nanoscale chemical and structural information about these processes ...
Gea T. van de Kerkhof +10 more
openaire +2 more sources
Research on the simulation environment of hard X-ray nanoprobe beamline station
Abstract In the hard X-ray nanoprobe beamline station, experiments need to be performed by adjusting the optical equipment in order to obtain good beamline performance. Due to many factors, only through the use of intelligent optimization, the beamline performance can be quickly improved.
Yuhao Liu +4 more
openaire +1 more source
The Velociprobe: An ultrafast hard X-ray nanoprobe for high-resolution ptychographic imaging [PDF]
Motivated by the advanced photon source upgrade, a new hard X-ray microscope called “Velociprobe” has been recently designed and built for fast ptychographic imaging with high spatial resolution. We are addressing the challenges of high-resolution and fast scanning with novel hardware designs, advanced motion controls, and new data acquisition ...
Junjing Deng +18 more
openaire +2 more sources
Beam and sample movement compensation for robust spectro-microscopy measurements on a hard X-ray nanoprobe [PDF]
Static and in situ nanoscale spectro-microscopy is now routinely performed on the Hard X-ray Nanoprobe beamline at Diamond and the solutions implemented to provide robust energy scanning and experimental operation are described. A software-based scheme for active feedback stabilization of X-ray beam position and monochromatic beam flux across the ...
Paul D. Quinn +3 more
openaire +2 more sources
Fluorescence imaging ofDictyostelium discoideumwith a hard X-ray nanoprobe
The preparation and a novel sample environment for X-ray based imaging of freeze-dried Dictyostelium discoideum cells are presented. As a first application a fluorescence imaging experiment with a nanofocused hard X-ray beam has been performed. The successful preparation was verified in elemental mappings with sub-200nm resolution, which allowed for ...
Tucoulou, R. +9 more
openaire +2 more sources
Three-Dimensional Correlative Imaging of a Malaria-Infected Cell with a Hard X-ray Nanoprobe [PDF]
Benefiting from the recent advances of synchrotron X-ray nanoprobes, we demonstrate three-dimensional (3D) correlative nanoimaging on malaria-infected human red blood cells. By combining X-ray fluorescence tomography and phase contrast nanotomography on the same cell with sub-100 nm pixel size, we establish a routine workflow from the data acquisition,
Y. Yang +7 more
openaire +4 more sources
Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses.
Simone Sala +9 more
openaire +4 more sources
AI‐based tools enable rapid characterization of bacterial ultrastructure in low‐dose cryogenic transmission electron microscopy. The envelope thickness tool quantifies membrane thickness and anisotropy. The flagella module analyzes filament morphology and detects cell‐flagella contacts.
Sita Sirisha Madugula +10 more
wiley +1 more source
Autonomous X‐Ray Fluorescence Mapping for Nanoscale Chemical Speciation of Fine Particulate Matter
We present X‐AutoMap, an autonomous X‐ray fluorescence mapping framework that integrates real‐time analysis with rule‐based computer vision to selectively target chemically relevant regions. By avoiding background‐dominated areas, the method reduces acquisition time by fourfold while enabling accurate particle‐level speciation.
Carlos Deleon +3 more
wiley +1 more source
Fluorescent Hydrogel‐Based Strain Sensor With Machine Learning‐Augmented Performance
Fluorescent hydrogel strain sensor based on carbon quantum dots enabling optical readout of deformation through strain‐dependent emission changes, coupled with Random Forest analysis to capture nonlinear fluorescence‐concentration relationships and identify optimal sensing conditions. Hydrogels are ideal matrices for bio‐integrated wearable sensors due
Tailai Chen +4 more
wiley +1 more source

